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IGF1R beta antibody

KO/KD Orthogonal
Anti-IGF1R beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50433
Anti-IGF1R beta antibody used in Western Blot (WB). GTX50433
Anti-IGF1R beta antibody used in IHC (Paraffin sections) (IHC-P). GTX50433
Anti-IGF1R beta antibody used in Western Blot (WB). GTX50433

Cat. No. GTX50433

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all IGF1R products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.1159~1163 (D-I-Y-E-T) derived from human IGF1R.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179335

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

Cellular Localization

Cell membrane

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-IGF1R beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50433

GTX50433 ICC/IF Image

Orthogonal

ICC/IF analysis of methanol-fixed MCF cells using GTX50433 IGF1R beta antibody.

Anti-IGF1R beta antibody used in Western Blot (WB). GTX50433

GTX50433 WB Image

Orthogonal

WB analysis of extracts from 293 cells using GTX50433 IGF1R beta antibody with or without blocking peptide.

Anti-IGF1R beta antibody used in IHC (Paraffin sections) (IHC-P). GTX50433

GTX50433 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50433 IGF1R beta antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-IGF1R beta antibody used in Western Blot (WB). GTX50433

GTX50433 WB Image

KO/KD

The data was published in the journal Sci Rep in 2020.PMID: 32427884

REFERENCE

Application Reference

Chiu YF et al. Sci Rep 2020; 10 (1) : 8261 Critical role of SOX2-IGF2 signaling in aggressiveness of bladder cancer.
Application : WB
Reactivity : Human
Wu SG et al. Cancers (Basel) 2019; 11 (1) IGFBP7 Drives Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibition in Lung Cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399