IGF1R beta antibody [HL1957]

Name: IGF1R beta antibody [HL1957]
Brand: GeneTex
SKU: GTX637794
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-IGF1R beta antibody [HL1957] used in Western Blot (WB). GTX637794

Anti-IGF1R beta antibody [HL1957] used in IHC (Paraffin sections) (IHC-P). GTX637794

Anti-IGF1R beta antibody [HL1957] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637794

Cat. No. GTX637794

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1957
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all IGF1R products

Datasheet

View product citations for antibody GTX637794 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:100-1:1000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Product Note

This antibody was raised against human IGF1R Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human IGF1R. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

Cellular Localization

Cell membrane

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Gene ID: 3480 IGF1R
UniProt: P08069 IGF1R

Research Area

DATA IMAGES

GTX637794 WB Image

Wild-type (WT) and IGF1R knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX637794 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637794 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX637794 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637794 IHC-P Image

IGF1R beta antibody [HL1957] detects IGF1R protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IGF1R beta stained by IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637794 ICC/IF Image

IGF1R beta antibody [HL1957] detects IGF1R beta protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF1R beta stained by IGF1R beta antibody [HL1957] (GTX637794) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

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