IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55013 IGF1R beta (phospho Tyr1131) / Insulin Receptor beta (phospho Tyr1146) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

WB analysis of IGF-treated HeLa (A) whole cell lysates using GTX55013 IGF1R beta (phospho Tyr1131) / Insulin Receptor beta (phospho Tyr1146) antibody.

ICC/IF analysis of formalin-fixed HeLa cells using GTX55013 IGF1R beta (phospho Tyr1131) / Insulin Receptor beta (phospho Tyr1146) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes