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IGF1R beta (phospho Tyr1131 / Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1146 / Tyr1150 / Tyr1151) antibody

Orthogonal
Anti-IGF1R beta (phospho Tyr1131 / Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1146 / Tyr1150 / Tyr1151) antibody used in Western Blot (WB). GTX25681

Cat. No. GTX25681

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Mouse, Rat, Chicken
Package
50 μl ($399)
See all Insulin Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Product Note

This antibody is raised against a synthetic peptide around phosphorylated Tyr1146 / Tyr1150 / Tyr1151 of Insulin Receptor (signal peptide cleaved form of P06213-2), which is identical to the sequence around phosphorylated Tyr1131 / Tyr1135 / Tyr1136 of IGF1R (signal peptide cleaved form).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosines 1146, 1150 and 1151 of the human insulin receptor. The corresponding residues in the IGF1R are 1131, 1135 and 1136.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380649

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor , CD220 , HHF5

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-IGF1R beta (phospho Tyr1131 / Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1146 / Tyr1150 / Tyr1151) antibody used in Western Blot (WB). GTX25681

GTX25681 WB Image

Orthogonal

WB analysis of samples using GTX25681 IGF1R beta (phospho Tyr1131 / Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1146 / Tyr1150 / Tyr1151) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

Application Reference

Zuliani I et al. Neurotherapeutics 2020; (Epub) The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome.
Application : WB
Reactivity : Mouse
Tramutola A et al. Neurobiol Dis 2020; 137 : 104772 Brain insulin resistance triggers early onset Alzheimer disease in Down syndrome.
Application : WB
Reactivity : Human
Chen YT et al. Oncotarget 2016; (Epub) Inhibition of DNA methyltransferase 1 increases nuclear receptor subfamily 4 group A member 1 expression and decreases blood glucose in type 2 diabetes.
Application : WB
Reactivity : Human
Basso D et al. J Cell Physiol 2011; 226 (2) : 456-68 Altered intracellular calcium fluxes in pancreatic cancer induced diabetes mellitus: Relevance of the S100A8 N-terminal peptide (NT-S100A8).
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399