IGF1R beta (phospho Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1150 / Tyr1151) antibody

Cat. No. GTX50154

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX50154 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:200

Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Product Note

This antibody is raised against a synthetic peptide around phosphorylated Tyr1135 / Tyr1136 of IGF1R (signal peptide cleaved form), which is identical to the sequence around phosphorylated Tyr1150 / Tyr1151 of Insulin Receptor (signal peptide cleaved form of P06213-2).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 1135 / tyrosine 1136 (T-D-Y(p)-Y(p)-R-K) derived from human IGF1R.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

Cellular Localization

Cell membrane

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Gene ID: 3480 IGF1R
UniProt: P08069 IGF1R

Research Area

DATA IMAGES

GTX50154 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF cells using GTX50154 IGF1R beta (phospho Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1150 / Tyr1151) antibody.

GTX50154 WB Image

WB analysis of extracts from 293 cells untreated or treated with insulin using GTX50154 IGF1R beta (phospho Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1150 / Tyr1151) antibody.

REFERENCE

There are currently no references for IGF1R beta (phospho Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1150 / Tyr1151) antibody (GTX50154). Be the first to share your publications with this product.

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty