IGF1R beta (phospho Tyr1316) antibody [HL4099]

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R beta (phospho Tyr1136) / Insulin Receptor beta (phospho Tyr1151) antibody [HL4099] (GTX642545) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Specificity test was performed by coating the plate with recombinant IGF1R non-phospho peptide and IGF1R (phospho Tyr1316) peptide (15.63 ng/mL). Coated protein was probed with IGF1R beta (phospho Tyr1316) antibody [HL4099] (GTX642545) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.