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IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody

Anti-IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody used in Western Blot (WB). GTX133450

Cat No. GTX133450

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Positive Control

IGF-1 treated MCF-7 cell

Product Note

This antibody is raised against a synthetic peptide around phosphorylated Tyr1131 of IGF1R (signal peptide cleaved form), which is identical to the sequence around phosphorylated Tyr1146 of Insulin Receptor (signal peptide cleaved form of P06213-2).

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1131 of human IGF1R. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886990

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody used in Western Blot (WB). GTX133450

GTX133450 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (GTX133450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (GTX133450). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (GTX133450). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169