IGF1R beta (phospho Tyr1131) / Insulin Receptor beta (phospho Tyr1146) antibody

Cat. No. GTX55013

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399)

See all IGF1R products

Datasheet
Document

View product citations for antibody GTX55013 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
ICC/IF	1:100 - 1:500
IHC-P	1:100 - 1:200

Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Product Note

Tyr1161 in full-length IGF-1R is equivalent toTyr1131 in signal peptide cleaved IGF1R, and Tyr1185 in full-length Insulin Receptor is equivalent to Tyr1146 in signal peptide cleaved Insulin Receptor.

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of IGF1R. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

Cellular Localization

Cell membrane

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Gene ID: 3480 IGF1R
UniProt: P08069 IGF1R

Research Area

DATA IMAGES

GTX55013 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX55013 IGF1R beta (phospho Tyr1131) / Insulin Receptor beta (phospho Tyr1146) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX55013 WB Image

WB analysis of IGF-treated HeLa (A) whole cell lysates using GTX55013 IGF1R beta (phospho Tyr1131) / Insulin Receptor beta (phospho Tyr1146) antibody.

GTX55013 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55013 IGF1R beta (phospho Tyr1131) / Insulin Receptor beta (phospho Tyr1146) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty