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IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody

Anti-IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55013
Anti-IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody used in IHC (Paraffin sections) (IHC-P). GTX55013
Anti-IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody used in Western Blot (WB). GTX55013

Cat No. GTX55013

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Product Note

This antibody is raised against a synthetic peptide around phosphorylated Tyr1131 of IGF1R (signal peptide cleaved form), which is identical to the sequence around phosphorylated Tyr1146 of Insulin Receptor (signal peptide cleaved form of P06213-2).

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate pH7.3, 0.87% Sodium Chloride, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of IGF1R. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

Cellular Localization

Cell membrane

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55013

GTX55013 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX55013 IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody used in IHC (Paraffin sections) (IHC-P). GTX55013

GTX55013 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55013 IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody used in Western Blot (WB). GTX55013

GTX55013 WB Image

WB analysis of IGF-treated HeLa (A) whole cell lysates using GTX55013 IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody.

REFERENCE

There are currently no references for IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (GTX55013). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (GTX55013). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379