*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
This antibody is raised against a synthetic peptide around phosphorylated Tyr1150 / Tyr1151 of Insulin Receptor (signal peptide cleaved form of P06213-2), which is identical to the sequence around phosphorylated Tyr1135 / Tyr1136 of IGF1R (signal peptide cleaved form). Although exhibiting a preference for IR/IGF-1R, this antibody has been shown by both peptide competition and protein blotting to react with other dual phosphotyrosine motifs from proteins such as c-Met and Shc.
PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
Synthetic phosphopeptide derived from the region of Insulin Receptor that contains tyrosines 1150 and 1151. The corresponding residues in the IGF1R are 1135 and 1136.
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
insulin receptor , CD220 , HHF5
Cell membrane; Single-pass type I membrane protein
This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]