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IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody

Anti-IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25680
Anti-IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody used in Western Blot (WB). GTX25680

Cat No. GTX25680

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

Human, Mouse, Rat
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:500
IHC-P Assay dependent
FACS 1:20
IHC Assay dependent
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

Product Note

This antibody is raised against a synthetic peptide around phosphorylated Tyr1150 / Tyr1151 of Insulin Receptor (signal peptide cleaved form of P06213-2), which is identical to the sequence around phosphorylated Tyr1135 / Tyr1136 of IGF1R (signal peptide cleaved form). Although exhibiting a preference for IR/IGF-1R, this antibody has been shown by both peptide competition and protein blotting to react with other dual phosphotyrosine motifs from proteins such as c-Met and Shc.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide derived from the region of Insulin Receptor that contains tyrosines 1150 and 1151. The corresponding residues in the IGF1R are 1135 and 1136.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380648

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin receptor , CD220 , HHF5

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25680

GTX25680 ICC/IF Image

ICC/IF analysis of MCF7 cells treated with insulin (100nM for 5 min) using GTX25680 IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes

Anti-IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody used in Western Blot (WB). GTX25680

GTX25680 WB Image

WB (peptide competition) analysis of CHO-T cells over-expressing the human insulin receptor stimulated with 100 nM insulin for 10 min at 37ºC (Lane 2-5) using GTX25680 IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody prior incubated with the phosphopeptide immunogen (Lane 2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 3), or a generic phosphotyrosine-containing peptide (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

REVIEW

IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody Cat No. GTX25680

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 50 μg
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf