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IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody

Anti-IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody used in Western Blot (WB). GTX50154
Anti-IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50154

Cat No. GTX50154

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Specificity/Sensitivity

This antibody is raised against a synthetic peptide around phosphorylated Tyr1135 / Tyr1136 of IGF1R (signal peptide cleaved form), which is identical to the sequence around phosphorylated Tyr1150 / Tyr1151 of Insulin Receptor (signal peptide cleaved form of P06213-2).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 1135 / tyrosine 1136 (T-D-Y(p)-Y(p)-R-K) derived from human IGF1R.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

Cellular Localization

Cell membrane

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody used in Western Blot (WB). GTX50154

GTX50154 WB Image

WB analysis of extracts from 293 cells untreated or treated with insulin using GTX50154 IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody.

Anti-IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50154

GTX50154 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF cells using GTX50154 IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody.

REFERENCE

There are currently no references for IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody (GTX50154). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody (GTX50154). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319