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IGF1R (phospho Tyr950) antibody

Anti-IGF1R (phospho Tyr950) antibody used in Western Blot (WB). GTX133449

Cat No. GTX133449

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Positive Control

IGF-1 treated MCF-7 cell

Product Note

The full-length IGF1R includes the 30 aa signal peptide that is cleaved during maturation. The Tyr950 of mature IGF1R is equivalent to Tyr980 of uncleaved IGF1R.

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr980 of human IGF1R. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886989

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IGF1R (phospho Tyr950) antibody used in Western Blot (WB). GTX133449

GTX133449 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF1R (phospho Tyr980) antibody (GTX133449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for IGF1R (phospho Tyr950) antibody (GTX133449). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IGF1R (phospho Tyr950) antibody (GTX133449). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169