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IGF2 antibody [8H1]

Anti-IGF2 antibody [8H1] used in Western Blot (WB). GTX60630
Anti-IGF2 antibody [8H1] used in Flow cytometry (FACS). GTX60630
Anti-IGF2 antibody [8H1] used in ELISA (ELISA). GTX60630
Anti-IGF2 antibody [8H1] used in IHC (Paraffin sections) (IHC-P). GTX60630
Anti-IGF2 antibody [8H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60630
Anti-IGF2 antibody [8H1] used in IHC (Paraffin sections) (IHC-P). GTX60630

Cat. No. GTX60630

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8H1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all IGF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human IGF2 (AA: 25-180) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887990

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 2 , C11orf43 , GRDF , IGF-II , PP9974

Cellular Localization

Secreted

Background

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-IGF2 antibody [8H1] used in Western Blot (WB). GTX60630

GTX60630 WB Image

WB analysis of HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate using GTX60630 IGF2 antibody [8H1].

Anti-IGF2 antibody [8H1] used in Flow cytometry (FACS). GTX60630

GTX60630 FACS Image

FACS analysis of HepG2 cells using GTX60630 IGF2 antibody [8H1].
Green : IGF2
Red : negative control

Anti-IGF2 antibody [8H1] used in ELISA (ELISA). GTX60630

GTX60630 ELISA Image

ELISA analysis of antigen using GTX60630 IGF2 antibody [8H1].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-IGF2 antibody [8H1] used in IHC (Paraffin sections) (IHC-P). GTX60630

GTX60630 IHC-P Image

IHC-P analysis of ovarian cancer tissue using GTX60630 IGF2 antibody [8H1].

Anti-IGF2 antibody [8H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60630

GTX60630 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60630 IGF2 antibody [8H1].
Green : IGF2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-IGF2 antibody [8H1] used in IHC (Paraffin sections) (IHC-P). GTX60630

GTX60630 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60630 IGF2 antibody [8H1].

REFERENCE

Application Reference

Hsu CF et al. EBioMedicine 2019; 41 : 597-609 IGF-axis confers transformation and regeneration of fallopian tube fimbria epithelium upon ovulation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399