APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human IGF2 protein, and it does not cross react with human IGF1, INS-IGF2, and INS protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-term region of human IGF2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
insulin like growth factor 2 , C11orf43 , GRDF , IGF-II , PP9974
Cellular Localization
Secreted
Background
This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
Database
Research Area
DATA IMAGES
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/templates/default/js/lightbox/images/loading.gif "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
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GTX638104 WB Image
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638104 WB Image
Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638104 ELISA Image
Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL2132] (GTX638104) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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GTX638104 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for IGF2 antibody [HL2132] (GTX638104). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IGF2 antibody [HL2132] (GTX638104). Be the first to share your experience with this product.
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/small/GTX638104/GTX638104_44998_20230331_WB_Fraction_23041023_266.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/small/GTX638104/GTX638104_44998_20230414_WB_Ag_23041723_301.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104](/upload/website/prouct_img/small/GTX638104/GTX638104_44998_20230512_ELISA_Indirect_23051702_722.webp "Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/small/GTX638104/GTX638104_44998_20230526_WB_multiple_B_23053001_192.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230331_WB_Fraction_23041023_266.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230414_WB_Ag_23041723_301.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")
![Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104](/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230512_ELISA_Indirect_23051702_722.webp "Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104")
![Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104](/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230526_WB_multiple_B_23053001_192.webp "Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104")