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IGF2 antibody [HL2132]

Recombinant antibody Orthogonal Overexpression
Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104
Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104
Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104
Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104

Cat. No. GTX638104

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2132

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IGF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Product Note

This antibody is specific for human IGF2 protein, and it does not cross react with human IGF1, INS-IGF2, and INS protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-term region of human IGF2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 2 , C11orf43 , GRDF , IGF-II , PP9974

Cellular Localization

Secreted

Background

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-IGF2 antibody [HL2132] used in ELISA (ELISA). GTX638104

GTX638104 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL2132] (GTX638104) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104

GTX638104 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104

GTX638104 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGF2 antibody [HL2132] used in Western Blot (WB). GTX638104

GTX638104 WB Image

Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations