IGF2 antibody [N1C3]

Cat No. GTX100453

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse

See all IGF2 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

HepG2 , HepG2 conditioned medium

Predict Reactivity

Rat, Sheep, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Insulin Like Growth Factor 2,C11Orf43,Grdf,Igf-Ii,Pp9974,Igf2

Cellular Localization

Secreted

Background

This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq]

Database

Gene ID: 3481
UniProt: P01344

Research Area

DATA IMAGES

GTX100453 WB Image

HepG2 whole cell extract and conditioned medium (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] (GTX100453) diluted at 1:500.

GTX100453 IHC-P Image

IGF2 antibody [N1C3] detects IGF2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IGF2 antibody [N1C3] (GTX100453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100453 ICC/IF Image

IGF2 antibody [N1C3] detects IGF2 protein at cytoplasm by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min.
Green: IGF2 protein stained by IGF2 antibody [N1C3] (GTX100453) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf

Package	List Price ($)
100 μl	$ 319
25 μl	$ 169
Qty

Package

List Price ($)

100 μl

$ 319

25 μl

$ 169

Qty