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IGF2 antibody [N1C3]

Anti-IGF2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX100453
Anti-IGF2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100453
Anti-IGF2 antibody [N1C3] used in Western Blot (WB). GTX100453

Cat No. GTX100453

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all IGF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

HepG2 , HepG2 conditioned medium

Predict Reactivity

Rat, Sheep, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin like growth factor 2 , C11orf43 , GRDF , IGF-II , PP9974

Cellular Localization

Secreted

Background

This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IGF2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX100453

GTX100453 IHC-P Image

IGF2 antibody [N1C3] detects IGF2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IGF2 antibody [N1C3] (GTX100453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IGF2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100453

GTX100453 ICC/IF Image

IGF2 antibody [N1C3] detects IGF2 protein at cytoplasm by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min.
Green: IGF2 protein stained by IGF2 antibody [N1C3] (GTX100453) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-IGF2 antibody [N1C3] used in Western Blot (WB). GTX100453

GTX100453 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] (GTX100453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169