APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat, Sheep, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.35 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human IGF2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037229
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
insulin like growth factor 2 , C11orf43 , GRDF , IGF-II , PP9974
Cellular Localization
Secreted
Background
This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-IGF2 antibody [N1C3] used in Western Blot (WB). GTX100453](/templates/default/js/lightbox/images/loading.gif "Anti-IGF2 antibody [N1C3] used in Western Blot (WB). GTX100453")
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GTX100453 WB Image
HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] (GTX100453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100453 ICC/IF Image
IGF2 antibody [N1C3] detects IGF2 protein at cytoplasm by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min.
Green: IGF2 protein stained by IGF2 antibody [N1C3] (GTX100453) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
There are currently no references for IGF2 antibody [N1C3] (GTX100453). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IGF2 antibody [N1C3] (GTX100453). Be the first to share your experience with this product.
![Anti-IGF2 antibody [N1C3] used in Western Blot (WB). GTX100453](/upload/website/prouct_img/small/GTX100453/GTX100453_44517_20211231_WB_Fraction_22011718_370.jpg "Anti-IGF2 antibody [N1C3] used in Western Blot (WB). GTX100453")
![Anti-IGF2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100453](/upload/website/prouct_img/small/GTX100453/GTX100453_39995_20170412_IFA_19011506_144.jpg "Anti-IGF2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100453")
![Anti-IGF2 antibody [N1C3] used in Western Blot (WB). GTX100453](/upload/website/prouct_img/normal/GTX100453/GTX100453_44517_20211231_WB_Fraction_22011718_370.jpg "Anti-IGF2 antibody [N1C3] used in Western Blot (WB). GTX100453")
![Anti-IGF2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100453](/upload/website/prouct_img/normal/GTX100453/GTX100453_39995_20170412_IFA_19011506_144.jpg "Anti-IGF2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100453")