*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HepG2 , HepG2 conditioned medium
Rat, Sheep, Bovine, Dog, Pig(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human IGF2. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Insulin Like Growth Factor 2,C11Orf43,Grdf,Igf-Ii,Pp9974,Igf2
This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq]
GTX100453 WB Image
HepG2 whole cell extract and conditioned medium (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] (GTX100453) diluted at 1:500.
GTX100453 IHC-P Image
IGF2 antibody [N1C3] detects IGF2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IGF2 antibody [N1C3] (GTX100453) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX100453 ICC/IF Image
IGF2 antibody [N1C3] detects IGF2 protein at cytoplasm by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min.
Green: IGF2 protein stained by IGF2 antibody [N1C3] (GTX100453) diluted at 1:500.
Blue: Hoechst 33342 staining.