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IGF2 antibody

KO/KD Orthogonal Overexpression
Anti-IGF2 antibody used in Western Blot (WB). GTX129110
Anti-IGF2 antibody used in Western Blot (WB). GTX129110
Anti-IGF2 antibody used in Western Blot (WB). GTX129110
Anti-IGF2 antibody used in Western Blot (WB). GTX129110

Cat. No. GTX129110

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IGF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Predict Reactivity

Rabbit, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human IGF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885897

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 2 , C11orf43 , GRDF , IGF-II , PP9974

Cellular Localization

Secreted

Background

This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IGF2 antibody used in Western Blot (WB). GTX129110

GTX129110 WB Image

Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody (GTX129110) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGF2 antibody used in Western Blot (WB). GTX129110

GTX129110 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody (GTX129110) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGF2 antibody used in Western Blot (WB). GTX129110

GTX129110 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF2 antibody (GTX129110) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGF2 antibody used in Western Blot (WB). GTX129110

GTX129110 WB Image

KO/KD

The data was published in the journal Sci Rep in 2020.PMID: 32427884

REFERENCE

Application Reference

Chiu YF et al. Sci Rep 2020; 10 (1) : 8261 Critical role of SOX2-IGF2 signaling in aggressiveness of bladder cancer.
Application : WB
Reactivity : Human
Walendzik K et al. J Invest Dermatol 2019; (Epub) The Transcription Factor FOXN1 Regulates Skin Adipogenesis and Affects Susceptibility to Diet-Induced Obesity.
Shoji Y et al. J Surg Res 2019; 237 : 22-29 The Embryonic Stem Cell-Specific Transcription Factor ZFP57 Promotes Liver Metastasis of Colorectal Cancer.
Application : IHC-P
Reactivity : Human
Li X et al. Cancer Lett 2017; 407 : 45-56 LncRNAAC132217.4, a KLF8-regulated long non-coding RNA, facilitates oral squamous cell carcinoma metastasis by upregulating IGF2 expression.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169