GeneTex
United States (US)

IGF2BP1 antibody, Internal

GTX88062_1060_IHC-P_18121411_428.jpg
GTX88062_2536_WB_18121411_682.jpg

Cat No. GTX88062

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88062-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_006537.3; NP_001153895.1).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-EKVFAEHKISYSGQ, from the internal region of the protein sequence according to NP_006537.3; NP_001153895.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IMP1 Antibody , VICKZ1 Antibody , ZBP1 Antibody , CRDBP Antibody , CRD BP Antibody

Cellular Localization

Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Targeted to stress granules (SGs), but not processing bodies (PBs), during cellular stress. Colocalizes with G3BP1 and TIAL1 in SGs. Colocalizes with HIV-1 GAG at the cell edges. Found

Background

This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
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Database

Research Area

DATA IMAGES
GTX88062_1060_IHC-P_18121411_428.jpg

GTX88062 IHC-P Image

IHC-P analysis of human breast using GTX88062 IGF2BP1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88062_2536_WB_18121411_682.jpg

GTX88062 WB Image

WB analysis of K562 lysate using GTX88062 IGF2BP1 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer