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IGF2BP2 antibody [3F9]

Overexpression
Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306
Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306
Anti-IGF2BP2 antibody [3F9] used in Flow cytometry (FACS). GTX84306
Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306
Anti-IGF2BP2 antibody [3F9] used in Flow cytometry (FACS). GTX84306
Anti-IGF2BP2 antibody [3F9] used in Western Blot (WB). GTX84306
Anti-IGF2BP2 antibody [3F9] used in Flow cytometry (FACS). GTX84306
Anti-IGF2BP2 antibody [3F9] used in Western Blot (WB). GTX84306
Anti-IGF2BP2 antibody [3F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84306
Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306
Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306

Cat. No. GTX84306

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all IGF2BP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human IGF2BP2 (NP_006333) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 2 mRNA binding protein 2 , IMP-2 , IMP2 , VICKZ2

Cellular Localization

Nucleus,Cytoplasm

Background

Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.

Database

Research Area

DATA IMAGES

Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306

GTX84306 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84306 IGF2BP2 antibody [3F9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306

GTX84306 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84306 IGF2BP2 antibody [3F9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IGF2BP2 antibody [3F9] used in Flow cytometry (FACS). GTX84306

GTX84306 FACS Image

FACS analysis of HeLa cells using GTX84306 IGF2BP2 antibody [3F9].
Red : Primary antibody
Blue : Negative control antibody

Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306

GTX84306 IHC-P Image

IHC-P analysis of human liver tissue using GTX84306 IGF2BP2 antibody [3F9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IGF2BP2 antibody [3F9] used in Flow cytometry (FACS). GTX84306

GTX84306 FACS Image

FACS analysis of Jurkat cells using GTX84306 IGF2BP2 antibody [3F9].
Red : Primary antibody
Blue : Negative control antibody

Anti-IGF2BP2 antibody [3F9] used in Western Blot (WB). GTX84306

GTX84306 WB Image

Overexpression

WB analysis of HEK293T cells transfected with IGF2BP2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84306 IGF2BP2 antibody [3F9].
Loading : 5 ug per lane

Anti-IGF2BP2 antibody [3F9] used in Flow cytometry (FACS). GTX84306

GTX84306 FACS Image

FACS analysis of HEK293T cells transfected with either IGF2BP2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84306 IGF2BP2 antibody [3F9].

Anti-IGF2BP2 antibody [3F9] used in Western Blot (WB). GTX84306

GTX84306 WB Image

WB analysis of various cell lines using GTX84306 IGF2BP2 antibody [3F9].
Loading : 35 ug per lane

Anti-IGF2BP2 antibody [3F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84306

GTX84306 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with IGF2BP2 plasmid using GTX84306 IGF2BP2 antibody [3F9].

Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306

GTX84306 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84306 IGF2BP2 antibody [3F9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IGF2BP2 antibody [3F9] used in IHC (Paraffin sections) (IHC-P). GTX84306

GTX84306 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84306 IGF2BP2 antibody [3F9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519