GeneTex
United States (US)

IGF2R antibody [MEM-238]

IGF2-receptor-antibody-MEM-238-GTX28093-WB-1_18121411_604.jpg

Cat No. GTX28093

Host Mouse
Clonality Monoclonal
Clone Name MEM-238
Isotype IgG1
Application WB, FACS
Reactivity Human, Mouse, Primate
APPLICATION

Application Note

Recommended Starting Dilutions:

For FACS: Use at an assay dependent dilution.
For WB (Non-reducing): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

250 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Vaccinia-M6PR/iGFIIR

Purification

Protein A affinity purified
> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Insulin Like Growth Factor 2 Receptor,Cd222,Ci-M6Pr,Cimpr,M6P-R,M6P/Igf2R,Mpr 300,Mpr1,Mpr300,Mpri,Igf2R

Cellular Localization

Late endosomal/prelysosomal compartment

Background

CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].

Database

Research Area

DATA IMAGES
IGF2-receptor-antibody-MEM-238-GTX28093-WB-1_18121411_604.jpg

GTX28093 WB Image

Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line.
Lane 1: immunostaining with anti-CD222 [MEM-238]
Lane 2: immunostaining with Isotype mouse IgG1 control

REFERENCE
Package List Price ($)
$ 319