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IGFBP2 antibody

Anti-IGFBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113471
Anti-IGFBP2 antibody used in Western Blot (WB). GTX113471
Anti-IGFBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113471
Anti-IGFBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113471

Cat. No. GTX113471

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IGFBP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGFBP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729300

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor binding protein 2 , IBP2 , IGF-BP53

Cellular Localization

Secreted

Background

Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Database

Research Area

DATA IMAGES

Anti-IGFBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113471

GTX113471 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using IGFBP2(GTX113471) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IGFBP2 antibody used in Western Blot (WB). GTX113471

GTX113471 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX113471 diluted at 1:1000

Anti-IGFBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113471

GTX113471 IHC-P Image

IGFBP2 antibody detects secreted IGFBP2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
IGFBP2 stained by IGFBP2 antibody (GTX113471) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IGFBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113471

GTX113471 IHC-P Image

The data was published in the journal PLoS One in 2015. PMID: 25978399

REFERENCE

Application Reference

Ehlken C et al. PLoS One 2015; 10 (5) : e0126859 Increased expression of angiogenic and inflammatory proteins in the vitreous of patients with ischemic central retinal vein occlusion.
Chen JY et al. J Nutr Biochem 2013; 24 (11) : 1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169