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IGFBP2 antibody

Anti-IGFBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128977
Anti-IGFBP2 antibody used in Western Blot (WB). GTX128977
Anti-IGFBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128977

Cat. No. GTX128977

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IGFBP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

SK-N-AS

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGFBP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885858

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor binding protein 2 , IBP2 , IGF-BP53

Cellular Localization

Secreted

Background

Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Database

Research Area

DATA IMAGES

Anti-IGFBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128977

GTX128977 ICC/IF Image

IGFBP2 antibody detects IGFBP2 protein at cytoplasm by immunofluorescent analysis.
Sample: Huh-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGFBP2 protein stained by IGFBP2 antibody (GTX128977) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-IGFBP2 antibody used in Western Blot (WB). GTX128977

GTX128977 WB Image

IGFBP2 antibody detects IGFBP2 protein by western blot analysis.
A. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
IGFBP2 antibody (GTX128977) dilution: 1:1000

Anti-IGFBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128977

GTX128977 IHC-P Image

IGFBP2 antibody detects IGFBP2 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IGFBP2 antibody (GTX128977) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lee CC et al. PLoS One 2017; 12 (3) : e0173890 the microrna-302b-inhibited *-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor ia.
Application : WB
Reactivity : Human
Chen PH et al. Neuropharmacology 2016; 110 (Pt A) : 362-75 The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169