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IGFBP3 antibody [N2C3]

Anti-IGFBP3 antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX100454
Anti-IGFBP3 antibody [N2C3] used in Western Blot (WB). GTX100454
Anti-IGFBP3 antibody [N2C3] used in Western Blot (WB). GTX100454

Cat No. GTX100454

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , mouse plasma

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGFBP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin like growth factor binding protein 3 , BP-53 , IBP3

Cellular Localization

Secreted

Background

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IGFBP3 antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX100454

GTX100454 IHC-Fr Image

IGFBP3 antibody [N2C3] detects IGFBP3 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: IGFBP3 protein stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IGFBP3 antibody [N2C3] used in Western Blot (WB). GTX100454

GTX100454 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
12% SDS PAGE
GTX100454 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGFBP3 antibody [N2C3] used in Western Blot (WB). GTX100454

GTX100454 WB Image

Mouse plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody (GTX100454) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169