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IGSF8 antibody

Anti-IGSF8 antibody used in IHC (Paraffin sections) (IHC-P). GTX00745
Anti-IGSF8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00745
Anti-IGSF8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00745
Anti-IGSF8 antibody used in Western Blot (WB). GTX00745
Anti-IGSF8 antibody used in Western Blot (WB). GTX00745

Cat No. GTX00745

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Mouse
Package
100 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100
IHC-P 1:100
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4), 50% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Full-length mouse IGSF8 with Flag tag

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

immunoglobulin superfamily, member 8 , ESTM34 , EWI-2 , KCT-4 , PGRL

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-IGSF8 antibody used in IHC (Paraffin sections) (IHC-P). GTX00745

GTX00745 IHC-P Image

IHC-P analysis of mouse lung tissue section using GTX00745 IGSF8 antibody.
Dilution : 1:100
Antigen retrieval : Proteolytic-Induced Epitope Retrieval

Anti-IGSF8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00745

GTX00745 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX00745 IGSF8 antibody.
Dilution : 1:100
Fixation : 4% PFA (v/v)

Anti-IGSF8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00745

GTX00745 ICC/IF Image

ICC/IF analysis of eggs of wild-type mouse or Igsf8 knock-out mouse using GTX00745 IGSF8 antibody. Zona-free eggs were fixed in PBS containing 0.5% (v/v) polyvinylpyrrolidone and 4% (v/v) paraformaldehyde.
Dilution : 1:100

Anti-IGSF8 antibody used in Western Blot (WB). GTX00745

GTX00745 WB Image

WB analysis of NIH-3T3 whole cell lysate using GTX00745 IGSF8 antibody.
Dilution : 1:1000
Loading : 40 μg
SDS-PAGE : 12.5%

Anti-IGSF8 antibody used in Western Blot (WB). GTX00745

GTX00745 WB Image

WB analysis of various mouse tissue samples of Igff8-targeted mice using GTX00745 IGSF8 antibody.
(F) : floxed alleles
(-) : knock-out alleles
Dilution : 1:500
Loading : 30 μg

REFERENCE

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REVIEW

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