WB analysis of NIH-3T3 cell lysate using GTX12134 IKB alpha antibody [6A920].

WB analysis of (A) Daudi and (B) NIH 3T3 cell lysate using GTX12134 IKB alpha antibody [6A920].
Dilution : 2 μg/ml

WB analysis of 100 μg/ml ALLN* (N-Acetyl-Leu-Leu-Norleucinal) treated Jurkat cell followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3) using GTX12134 IKB alpha antibody [6A920] and IKB alpha phospho-specific antibody.
*ALLN (N-Acetyl-Leu-Leu-Norleucinal) : Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation

FACS (Intracellular staining) analysis of THP-1 cells using GTX12134 IKB alpha antibody [6A920].
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Antibody amount : 0.25μg/10^6 cells

WB analysis of Jurkat, LNCaP, PC-3, HeLa, NIH3T3 cell lysates using GTX12134 IKB alpha antibody [6A920].
Dilution : 0.5 μg/ml

IHC-P analysis of human breast tissue using GTX12134 IKB alpha antibody [6A920].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0