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IKB alpha antibody

Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Anti-IKB alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110521
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Anti-IKB alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110521
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

Cat No. GTX110521

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HT-29 , NIH-3T3 , BCL-1 , C2C12 , *MCF7 , *RAW264.7 , *B16F10

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377400)

Predict Reactivity

Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IKB alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NFKB inhibitor alpha,EDAID2,IKBA,MAD-3,NFKBI

Cellular Localization

Cytoplasm , Nucleus

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

GTX110521 WB Image

Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKB alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110521

GTX110521 IHC-P Image

IKB alpha antibody detects IKB alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IKB alpha stained by IKB alpha antibody (GTX110521) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

GTX110521 WB Image

IKB alpha antibody detects IKB alpha protein by western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extrac
10% SDS-PAGE
IKB alpha antibody (GTX110521) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKB alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110521

GTX110521 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha(GTX110521) antibody at 1:200 dilution.

Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

GTX110521 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

GTX110521 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

IKB alpha antibody Cat No. GTX110521

Rating ( Average 4 based on 7 users reviews)
Application
Western Blot(WB) ( Average 4 based on 7 users reviews)
Date : Anonymous submitted on 22-Mar-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : mpkCCD cells
Amount used : 70 μg
Blocking : 0.1%BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:2500, 4°C, 16Hr
Date : Anonymous submitted on 10-Mar-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 15 μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : mpkCCD cells
Amount used : 60μg
Blocking : 0.1% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Amount used : 30μg
Blocking : 5% skim milk in PBST, 25°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : B16F10 cells
Amount used : 30μg
Blocking : 5% skim milk in PBST, 25°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 01-Jun-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW 264.7 cells
Amount used : 60μg
Blocking : 5% skim milk in TBST, 4°C, overnightHr
Primary Antibody : 1:1000, 25°C, 2Hr
Date : Anonymous submitted on 25-May-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 50μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf