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IKB alpha antibody

KO/KD Comparison IP/MS
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Anti-IKB alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110521
Anti-IKB alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110521
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

Cat. No. GTX110521

Reference 13
8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Pig
Package
100 μl ($399),
25 μl ($169)
See all IKB alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

BCL-1 , C2C12 , NIH-3T3 , HT-29 , 293T , A431 , HeLa , HepG2

Product Note

IP/MS validation was supported by references (PMID:30377400)

Predict Reactivity

Rat, Sheep, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IKB alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950996

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI

Cellular Localization

Cytoplasm , Nucleus

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

GTX110521 WB Image

KO/KD

Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

GTX110521 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with IKB alpha antibody (GTX110521) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

GTX110521 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

GTX110521 WB Image

IKB alpha antibody detects IKB alpha protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extrac
10% SDS-PAGE
IKB alpha antibody (GTX110521) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKB alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110521

GTX110521 IHC-P Image

IKB alpha antibody detects IKB alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IKB alpha stained by IKB alpha antibody (GTX110521) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IKB alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110521

GTX110521 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha(GTX110521) antibody at 1:200 dilution.

Anti-IKB alpha antibody used in Western Blot (WB). GTX110521

GTX110521 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Vichuda Charoensaensuk et al. Antioxidants (Basel) 2022; 11 (4) 2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside Attenuates Reactive Oxygen Species-Dependent Inflammation and Apoptosis in Porphyromonas gingivalis-Infected Brain Endothelial Cells.
Application : WB
Yao L et al. Front Cell Dev Biol 2021; 9 : 685913 Toxoplasma gondii Type-I ROP18 Targeting Human E3 Ligase TRIM21 for Immune Escape.
Application : WB
Yang D et al. Int Immunopharmacol 2021; 90 : 107149 Mitoquinone ameliorates cigarette smoke-induced airway inflammation and mucus hypersecretion in mice.
Application : WB
Reactivity : Mouse
Feng JY et al. Microbes Infect 2020; TREM-1 enhances Mycobacterium tuberculosis-induced inflammatory responses in macrophages.
Application : WB
Reactivity : Mouse
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

IKB alpha antibody Cat. No. GTX110521

Rating
( Average 4.1 based on 8 users reviews)
Application
Western Blot(WB)
( Average 4.1 based on 8 users reviews)
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Date : Anonymous submitted on 01-May-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 cells
Amount used : 25μg
Blocking : 5% non-fat milk in TBST, 22°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Date : Anonymous submitted on 22-Mar-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : mpkCCD cells
Amount used : 70 μg
Blocking : 0.1%BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:2500, 4°C, 16Hr
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Date : Anonymous submitted on 10-Mar-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 15 μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Date : Anonymous submitted on 23-Aug-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : mpkCCD cells
Amount used : 60μg
Blocking : 0.1% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Amount used : 30μg
Blocking : 5% skim milk in PBST, 25°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : B16F10 cells
Amount used : 30μg
Blocking : 5% skim milk in PBST, 25°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Date : Anonymous submitted on 01-Jun-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW 264.7 cells
Amount used : 60μg
Blocking : 5% skim milk in TBST, 4°C, overnightHr
Primary Antibody : 1:1000, 25°C, 2Hr
Anti-IKB alpha antibody used in Western Blot (WB). GTX110521
Date : Anonymous submitted on 25-May-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 50μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Vichuda Charoensaensuk et al. Antioxidants (Basel) 2022; 11 (4) 2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside Attenuates Reactive Oxygen Species-Dependent Inflammation and Apoptosis in Porphyromonas gingivalis-Infected Brain Endothelial Cells.
Application : WB
Yao L et al. Front Cell Dev Biol 2021; 9:685913 Toxoplasma gondii Type-I ROP18 Targeting Human E3 Ligase TRIM21 for Immune Escape.
Application : WB
Yang D et al. Int Immunopharmacol 2021; 90:107149 Mitoquinone ameliorates cigarette smoke-induced airway inflammation and mucus hypersecretion in mice.
Application : WB
Reactivity : Mouse
Feng JY et al. Microbes Infect 2020; TREM-1 enhances Mycobacterium tuberculosis-induced inflammatory responses in macrophages.
Application : WB
Reactivity : Mouse
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
Application : WB
Reactivity : Human
Hsiao HT et al. J Biomed Sci 2019; 26 (1):74 The analgesic effect of propofol associated with the inhibition of hypoxia inducible factor and inflammasome in complex regional pain syndrome.
Application : WB
Reactivity : Mouse
Xie MY et al. J Agric Food Chem 2019; 67 (34):9477-9491 Porcine Milk Exosome MiRNAs Attenuate LPS-Induced Apoptosis through Inhibiting TLR4/NF-庥B and p53 Pathways in Intestinal Epithelial Cells.
Application : WB
Reactivity : Pig
Wang Z et al. Int Immunopharmacol 2019; 76:105873 Butylphthalide ameliorates airway inflammation and mucus hypersecretion via NF-庥B in a murine asthma model.
Wang X et al. Scand J Immunol 2019;:e12791 Epoxyeicosatrienoic acids alleviate methionine-choline-deficient diet-induced non-alcoholic steatohepatitis in mice.
Application : WB
Reactivity : Mouse
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Lee HM et al. Transplant Proc 2016; 48 (4):1226-33 Protective Effect of Eupatilin Pretreatment Against Hepatic Ischemia-Reperfusion Injury in Mice.
Previtera ML et al. PLoS One 2015; 10 (12):e0145813 Substrate Stiffness Regulates Proinflammatory Mediator Production through TLR4 Activity in Macrophages.
Application : WB
Reactivity : Mouse
Yang DJ et al. J Agric Food Chem 2014; 62 (15):3458-65 Inhibitory effect of litchi (Litchi chinensis Sonn.) flower on lipopolysaccharide-induced expression of proinflammatory mediators in RAW264.7 cells through NF-κB, ERK, and JAK2/STAT3 inactivation.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169