GeneTex
United States (US)

IKB alpha antibody

Cat No. GTX27545

Host Rabbit
Clonality Polyclonal
Application WB, ELISA, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000 - 1:10000
ELISA 1:20000 - 1:100000
IHC 1:1000 - 1:5000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.1% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

80 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IKB alpha Antibody was produced by repeated immunizations with a synthetic IkBa peptide corresponding to a region near the C-terminus of the human protein.

Purification

Antiserum
This product was prepared from monospecific antiserum by delipidation and defibrination.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nfkb Inhibitor Alpha,Ikba,Mad-3,Nfkbi,Nfkbia

Cellular Localization

Cytoplasmic

Background

Three major forms of IkB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkB-alpha and IkB-beta appear to be the major regulatory forms of IkB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkB and block nuclear import by masking the nuclear localization sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkB. Immunoblotting easily detects the hyper-phosphorylated forms of IkB-alpha, but not phosphorylated IkB-beta. Interestingly, IkB-alpha and IkB-beta mediate different NFkB responses. IkB-alpha appears to control more transient activation of NFkB in response to an inducer, while IkB-beta controls a persistent response. Bcl-3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkB is confirmed by immunoblotting.

Database

Research Area

Package List Price ($)
$ 319