GeneTex
United States (US)

IKB alpha (phospho Ser32/Ser36) antibody

GTX32224_20190131_WB_19013101_520.jpg
GTX32224_20190131_ICCIF_19013101_782.jpg
GTX32224_20190131_IHC-P_19013101_451.jpg

Cat No. GTX32224

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of IKB alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nfkb Inhibitor Alpha,Ikba,Mad-3,Nfkbi,Nfkbia

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
GTX32224_20190131_WB_19013101_520.jpg

GTX32224 WB Image

WB analysis of TNFa-treated HepG2 (A), TNFa-treated Raw264.7 (B), TNFa-treated PC12 (C) whole cell lysates using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody.

GTX32224_20190131_ICCIF_19013101_782.jpg

GTX32224 ICC/IF Image

ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32224_20190131_IHC-P_19013101_451.jpg

GTX32224 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Package List Price ($)
$ 319