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IKB alpha (phospho Ser32/Ser36) antibody

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in IHC (Paraffin sections) (IHC-P). GTX32224
Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32224
Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Western Blot (WB). GTX32224

Cat. No. GTX32224

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all IKB alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of IKB alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887643

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in IHC (Paraffin sections) (IHC-P). GTX32224

GTX32224 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32224

GTX32224 ICC/IF Image

ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Western Blot (WB). GTX32224

GTX32224 WB Image

WB analysis of TNFa-treated HepG2 (A), TNFa-treated Raw264.7 (B), TNFa-treated PC12 (C) whole cell lysates using GTX32224 IKB alpha (phospho Ser32/Ser36) antibody.

REFERENCE

Application Reference

Hu Y et al. Mol Ther Nucleic Acids 2020; 19 : 1330-1342 Downregulation of Hypoxia-Inducible Factor-1α by RNA Interference Alleviates the Development of Collagen-Induced Arthritis in Rats.
Gao F et al. Adv Sci (Weinh) 2019; 6 (7) : 1801671 Au Clusters Treat Rheumatoid Arthritis with Uniquely Reversing Cartilage/Bone Destruction.
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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399