GeneTex
  • Country / Location Selection

United States (US)

IKB alpha (phospho Ser32/Ser36) antibody

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Western Blot (WB). GTX50209
Anti-IKB alpha (phospho Ser32/Ser36) antibody used in IHC (Paraffin sections) (IHC-P). GTX50209
Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50209

Cat No. GTX50209

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from human IKB alpha.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Western Blot (WB). GTX50209

GTX50209 WB Image

WB analysis of extracts from HeLa cells untreated or treated with TNF using GTX50209 IKB alpha (phospho Ser32/Ser36) antibody.

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in IHC (Paraffin sections) (IHC-P). GTX50209

GTX50209 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50209 IKB alpha (phospho Ser32/Ser36) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50209

GTX50209 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50209 IKB alpha (phospho Ser32/Ser36) antibody.

REFERENCE

REVIEW

There are currently no reviews for IKB alpha (phospho Ser32/Ser36) antibody (GTX50209). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319