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IKB alpha (phospho Ser32/Ser36) antibody

Orthogonal
Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50209
Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Western Blot (WB). GTX50209
Anti-IKB alpha (phospho Ser32/Ser36) antibody used in IHC (Paraffin sections) (IHC-P). GTX50209

Cat. No. GTX50209

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all IKB alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from human IKB alpha.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11174813

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50209

GTX50209 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50209 IKB alpha (phospho Ser32/Ser36) antibody.

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in Western Blot (WB). GTX50209

GTX50209 WB Image

Orthogonal

WB analysis of extracts from HeLa cells untreated or treated with TNF using GTX50209 IKB alpha (phospho Ser32/Ser36) antibody.

Anti-IKB alpha (phospho Ser32/Ser36) antibody used in IHC (Paraffin sections) (IHC-P). GTX50209

GTX50209 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50209 IKB alpha (phospho Ser32/Ser36) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Er-Chieh Cho et al. Mediators of Inflammation 2015; RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399