Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha (phospho Ser32) antibody [GT1205] (GTX00967) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of C6 cell extracts with/without TNF-α (20 ng/ml, 37ºC, 30min) treatment using GTX00967 IKB alpha (phospho Ser32) antibody [GT1205].
Dilution : 1:1000
Loading : 25μg

WB analysis of NIH-3T3 cell extracts with/without TNF-α (20 ng/ml, 37ºC, 30min) treatment using GTX00967 IKB alpha (phospho Ser32) antibody [GT1205].
Dilution : 1:1000
Loading : 25μg