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IKB alpha (phospho Ser32) antibody [GT1205]

Recombinant antibody Orthogonal
Anti-IKB alpha (phospho Ser32) antibody [GT1205] used in Western Blot (WB). GTX00967
Anti-IKB alpha (phospho Ser32) antibody [GT1205] used in Western Blot (WB). GTX00967
Anti-IKB alpha (phospho Ser32) antibody [GT1205] used in Western Blot (WB). GTX00967

Cat. No. GTX00967

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1205

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all IKB alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Phospho-IKB alpha (S32).

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB inhibitor alpha , IKBA , MAD3 , NFKBI , NFKBIA , IKB alpha , IkappaB-alpha , NFkb inhibitor alpha , NF-kappa-B inhibitor alpha

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IKB alpha (phospho Ser32) antibody [GT1205] used in Western Blot (WB). GTX00967

GTX00967 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha (phospho Ser32) antibody [GT1205] (GTX00967) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKB alpha (phospho Ser32) antibody [GT1205] used in Western Blot (WB). GTX00967

GTX00967 WB Image

WB analysis of NIH-3T3 cell extracts with/without TNF-α (20 ng/ml, 37ºC, 30min) treatment using GTX00967 IKB alpha (phospho Ser32) antibody [GT1205].
Dilution : 1:1000
Loading : 25μg

Anti-IKB alpha (phospho Ser32) antibody [GT1205] used in Western Blot (WB). GTX00967

GTX00967 WB Image

WB analysis of C6 cell extracts with/without TNF-α (20 ng/ml, 37ºC, 30min) treatment using GTX00967 IKB alpha (phospho Ser32) antibody [GT1205].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399