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IKB alpha (phospho Ser32) antibody [ST53-05]

Anti-IKB alpha (phospho Ser32) antibody [ST53-05] used in Western Blot (WB). GTX00976
Anti-IKB alpha (phospho Ser32) antibody [ST53-05] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00976
Anti-IKB alpha (phospho Ser32) antibody [ST53-05] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00976

Cat No. GTX00976

Host

Rabbit

Clonality

Monoclonal

Clone Name

ST53-05

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NFKB inhibitor alpha,IKBA,MAD3,NFKBI,NFKBIA,IKB alpha,IkappaB-alpha,NFkb inhibitor alpha,NF-kappa-B inhibitor alpha

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IKB alpha (phospho Ser32) antibody [ST53-05] used in Western Blot (WB). GTX00976

GTX00976 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with IKB alpha (phospho Ser32) antibody [ST53-05] (GTX00976) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IKB alpha (phospho Ser32) antibody [ST53-05] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00976

GTX00976 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00976 IKB alpha (phospho Ser32) antibody [ST53-05].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-IKB alpha (phospho Ser32) antibody [ST53-05] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00976

GTX00976 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00976 IKB alpha (phospho Ser32) antibody [ST53-05].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for IKB alpha (phospho Ser32) antibody [ST53-05] (GTX00976). Be the first to share your publications with this product.

REVIEW

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