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IKB alpha (phospho Ser36) antibody [GT1298]

Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in Western Blot (WB). GTX03210
Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in IHC (Paraffin sections) (IHC-P). GTX03210
Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in IHC (Paraffin sections) (IHC-P). GTX03210
Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in IHC (Paraffin sections) (IHC-P). GTX03210
Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in Western Blot (WB). GTX03210
Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in Western Blot (WB). GTX03210
Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in Western Blot (WB). GTX03210

Cat No. GTX03210

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1298

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S36 of human IκBα.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in Western Blot (WB). GTX03210

GTX03210 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha (phospho Ser36) antibody [GT1298] (GTX03210) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in IHC (Paraffin sections) (IHC-P). GTX03210

GTX03210 IHC-P Image

IHC-P analysis of rat ovary tissue section using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:100

Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in IHC (Paraffin sections) (IHC-P). GTX03210

GTX03210 IHC-P Image

IHC-P analysis of mouse liver tissue section using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:100

Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in IHC (Paraffin sections) (IHC-P). GTX03210

GTX03210 IHC-P Image

IHC-P analysis of human colon tissue section using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:100

Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in Western Blot (WB). GTX03210

GTX03210 WB Image

WB analysis of C6 cells were treated by TNF-α (20 ng/mL) at 37ºC for 30 minutes using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:1000
Loading : 25μg per lane

Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in Western Blot (WB). GTX03210

GTX03210 WB Image

WB analysis of THP-1 cells were treated by PMA/TPA (80 nM) at 37ºC for overnight or treated by LPS (1 μg/mL) at 37ºC for 6 hours using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:1000
Loading : 25μg per lane

Anti-IKB alpha (phospho Ser36) antibody [GT1298] used in Western Blot (WB). GTX03210

GTX03210 WB Image

WB analysis of NIH-3T3 cells were treated by TNF-α (20 ng/mL) at 37ºC for 30 minutes using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

There are currently no references for IKB alpha (phospho Ser36) antibody [GT1298] (GTX03210). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IKB alpha (phospho Ser36) antibody [GT1298] (GTX03210). Be the first to share your experience with this product.
Package List Price ($)
$ 379