Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha (phospho Ser36) antibody [GT1298] (GTX03210) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of THP-1 cells were treated by PMA/TPA (80 nM) at 37ºC for overnight or treated by LPS (1 μg/mL) at 37ºC for 6 hours using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:1000
Loading : 25μg per lane

IHC-P analysis of human colon tissue section using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:100

IHC-P analysis of mouse liver tissue section using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:100

WB analysis of C6 cells were treated by TNF-α (20 ng/mL) at 37ºC for 30 minutes using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:1000
Loading : 25μg per lane

IHC-P analysis of rat ovary tissue section using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:100

WB analysis of NIH-3T3 cells were treated by TNF-α (20 ng/mL) at 37ºC for 30 minutes using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:1000
Loading : 25μg per lane