IKB alpha (phospho Ser36) antibody [GT1298]

Cat. No. GTX03210

Reference 1

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1298
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($419)

See all IKB alpha products

Datasheet
Reference

View product citations for antibody GTX03210 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
IHC-P	1:50 - 1:200

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S36 of human IκBα.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Database

Gene ID: 4792 NFKBIA
UniProt: P25963 NFKBIA

Research Area

DATA IMAGES

GTX03210 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha (phospho Ser36) antibody [GT1298] (GTX03210) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX03210 WB Image

WB analysis of NIH-3T3 cells were treated by TNF-α (20 ng/mL) at 37ºC for 30 minutes using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:1000
Loading : 25μg per lane

GTX03210 IHC-P Image

IHC-P analysis of rat ovary tissue section using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:100

GTX03210 WB Image

WB analysis of C6 cells were treated by TNF-α (20 ng/mL) at 37ºC for 30 minutes using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:1000
Loading : 25μg per lane

GTX03210 IHC-P Image

IHC-P analysis of mouse liver tissue section using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:100

GTX03210 IHC-P Image

IHC-P analysis of human colon tissue section using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:100

GTX03210 WB Image

WB analysis of THP-1 cells were treated by PMA/TPA (80 nM) at 37ºC for overnight or treated by LPS (1 μg/mL) at 37ºC for 6 hours using GTX03210 IKB alpha (phospho Ser36) antibody [GT1298].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

L Liu et al. Pharm Biol 2024; 62 (1) : 607-620 Qing Hua Chang Yin ameliorates chronic colitis in mice by inhibiting PERK–ATF4–CHOP pathway of ER stress and the NF-κB signalling pathway

Submit a Reference

REVIEW

There are currently no reviews for IKB alpha (phospho Ser36) antibody [GT1298] (GTX03210). Be the first to share your experience with this product.

Submit a Review

Application Reference

L Liu et al. Pharm Biol 2024; 62 (1):607-620 Qing Hua Chang Yin ameliorates chronic colitis in mice by inhibiting PERK–ATF4–CHOP pathway of ER stress and the NF-κB signalling pathway

Package

List Price ($)

100 μl

$ 419

Qty

IKB alpha (phospho Ser36) antibody [GT1298]

Cat. No. GTX03210

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX03210 WB Image

GTX03210 WB Image

GTX03210 IHC-P Image

GTX03210 WB Image

GTX03210 IHC-P Image

GTX03210 IHC-P Image

GTX03210 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

© 1998-2022 GeneTex, Inc. All rights reserved.

All products are for research use only—Not for use in diagnostic or therapeutic applications.