IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32225 IKB beta (phospho Ser23) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

WB analysis of LPS-treated HEK293T (A), LPS-treated SP2/0 (B), LPS-treated PC12 (C), HT29 (D), TNFa-treated NIH3T3 (E) whole cell lysates using GTX32225 IKB beta (phospho Ser23) antibody.

ICC/IF analysis of formalin-fixed PC12 cells using GTX32225 IKB beta (phospho Ser23) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes