GeneTex
United States (US)

IKB beta (phospho Ser23) antibody

GTX32225_20190131_ICCIF_19013101_403.jpg
GTX32225_20190131_WB_19013101_325.jpg
GTX32225_20190131_IHC-P_19013101_951.jpg

Cat No. GTX32225

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of IKB beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nfkb Inhibitor Beta,Ikbb,Trip9,Nfkbib

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES
GTX32225_20190131_ICCIF_19013101_403.jpg

GTX32225 ICC/IF Image

ICC/IF analysis of formalin-fixed PC12 cells using GTX32225 IKB beta (phospho Ser23) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32225_20190131_WB_19013101_325.jpg

GTX32225 WB Image

WB analysis of LPS-treated HEK293T (A), LPS-treated SP2/0 (B), LPS-treated PC12 (C), HT29 (D), TNFa-treated NIH3T3 (E) whole cell lysates using GTX32225 IKB beta (phospho Ser23) antibody.

GTX32225_20190131_IHC-P_19013101_951.jpg

GTX32225 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32225 IKB beta (phospho Ser23) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)