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IKK alpha antibody [EPR464]

Anti-IKK alpha antibody [EPR464] used in IHC (Paraffin sections) (IHC-P). GTX62710
Anti-IKK alpha antibody [EPR464] used in Western Blot (WB). GTX62710

Cat. No. GTX62710

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 EPR464

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399)
See all IKK alpha products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1,000 - 10,000
For IHC: Use at a dilution of 1:100-250
For ICC: Use at a dilution of 1:50-100
For IP: Use at a dilution of 1:10-100
Optimal working dilution for a specific application should be determined by the investigator.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

50mM Tris-Glycine (pH7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to residues in human IKK alpha was used as an immunogen.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10621122

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,488.

TARGET

Synonyms

component of inhibitor of nuclear factor kappa B kinase complex , IKBKA , IKK-alpha , IKK1 , IKKA , NFKBIKA , TCF16

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IKK alpha antibody [EPR464] used in IHC (Paraffin sections) (IHC-P). GTX62710

GTX62710 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-IKK alpha RabMAb (cat. #GTX62710).

Anti-IKK alpha antibody [EPR464] used in Western Blot (WB). GTX62710

GTX62710 WB Image

Western blot analysis on (A) Jurkat (B) HeLa (C) HepG2 and (D) Daudi cell lysates using anti-IKK alpha RabMAb (cat. #GTX62710).

REFERENCE

Application Reference

Sharma RK et al. Immunol Invest 2017; 46 (8) : 833-846 Immunomodulatory activities of curcumin-stabilized silver nanoparticles: Efficacy as an antiretroviral therapeutic.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399