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IKK beta antibody [10A9B6]

Anti-IKK beta antibody [10A9B6] used in Western Blot (WB). GTX12140
Anti-IKK beta antibody [10A9B6] used in Immunoprecipitation (IP). GTX12140
Anti-IKK beta antibody [10A9B6] used in Flow cytometry (FACS). GTX12140
Anti-IKK beta antibody [10A9B6] used in Western Blot (WB). GTX12140
Anti-IKK beta antibody [10A9B6] used in Western Blot (WB). GTX12140

Cat No. GTX12140

Host

Mouse

Clonality

Monoclonal

Clone Name

10A9B6

Isotype

IgG1

Application

WB, ICC/IF, FACS, IP, Gel supershift assays

Reactivity

Human, Mouse
Package
50 μg ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 - 5 μg/ml
ICC/IF Assay dependent
FACS 1:10 - 1:1000
IP 2 - 5 μg/ 10⁶ cells
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human IKKb/IKK2 recombinant protein was used as immunogen (NP_001547).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase subunit beta , IKK-beta , IKK2 , IKKB , IMD15 , IMD15A , IMD15B , NFKBIKB

Cellular Localization

Cytoplasm,Nucleus

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IKK beta antibody [10A9B6] used in Western Blot (WB). GTX12140

GTX12140 WB Image

WB analysis of Daudi cell lysate using GTX12140 IKK beta antibody [10A9B6].
Dilution : 5 μg/ml

Anti-IKK beta antibody [10A9B6] used in Immunoprecipitation (IP). GTX12140

GTX12140 IP Image

IP analysis of Daudi cell lysate using GTX12140 IKK beta antibody [10A9B6].

Anti-IKK beta antibody [10A9B6] used in Flow cytometry (FACS). GTX12140

GTX12140 FACS Image

FACS (Intracellular staining) analysis of HEK293 cells using GTX12140 IKK beta antibody [10A9B6].
Red : primary antibody
Green : isotype control

Anti-IKK beta antibody [10A9B6] used in Western Blot (WB). GTX12140

GTX12140 WB Image

WB analysis of Daudi cells using GTX12140 IKK beta antibody [10A9B6].
Dilution : 5 μg/ml

Anti-IKK beta antibody [10A9B6] used in Western Blot (WB). GTX12140

GTX12140 WB Image

WB analysis of HeLa cell lysate using GTX12140 IKK beta antibody [10A9B6].

REFERENCE

There are currently no references for IKK beta antibody [10A9B6] (GTX12140). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IKK beta antibody [10A9B6] (GTX12140). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 259