APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2 - 4 μg/ml |
| Assay dependent |
| 1:10 - 1:500 |
| 0.25 - 0.5 μg / 10⁶ cells |
| 1:10 - 1:500 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
inhibitor of nuclear factor kappa B kinase subunit beta , IKK-beta , IKK2 , IKKB , IMD15 , IMD15A , IMD15B , NFKBIKB
Cellular Localization
Cytoplasm,Nucleus
Background
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139](/templates/default/js/lightbox/images/loading.gif "Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139")
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GTX12139 FACS Image
FACS analysis of HEK293 cells using GTX12139 IKK beta antibody [10AG2].
Red : primary antibody
Green : isotype control
Antibody amount : 0.1μg
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GTX12139 WB Image
WB analysis of (A) Daudi, B) HeLa, and C) NIH3T3 cell lysate using GTX12139 IKK beta antibody [10AG2].
Dilution : 2 μg/ml
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GTX12139 FACS Image
FACS (Intracellular staining) analysis of HeLa cells using GTX12139 IKK beta antibody [10AG2].
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA
Permibilization : 0.1% saponin
Antibody amount : 1μg/100 μl buffer
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GTX12139 FACS Image
FACS (Intracellular staining) analysis of Jurkat cells using GTX12139 IKK beta antibody [10AG2].
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA
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GTX12139 IHC-P Image
IHC-P analysis of human gallbladder tissue using GTX12139 IKK beta antibody [10AG2].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
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GTX12139 FACS Image
FACS (Intracellular staining) analysis of HEK293 cells using GTX12139 IKK beta antibody [10AG2].
Red : primary antibody
Green : isotype control
Antibody amount : 0.5μg
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GTX12139 WB Image
WB analysis of HeLa cell lysate using GTX12139 IKK beta antibody [10AG2].
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REFERENCE
There are currently no references for IKK beta antibody [10AG2] (GTX12139). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IKK beta antibody [10AG2] (GTX12139). Be the first to share your experience with this product.
![Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139](/upload/website/prouct_img/small/GTX12139/GTX12139_7_FACS_w_23060519_606.webp "Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139](/upload/website/prouct_img/small/GTX12139/GTX12139_926_WB_w_23060519_956.webp "Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139](/upload/website/prouct_img/small/GTX12139/GTX12139_63_FACS_w_23060519_346.webp "Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139](/upload/website/prouct_img/small/GTX12139/GTX12139_64_FACS_w_23060519_856.webp "Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139")
![Anti-IKK beta antibody [10AG2] used in IHC (Paraffin sections) (IHC-P). GTX12139](/upload/website/prouct_img/small/GTX12139/GTX12139_390_IHC-P_w_23060519_342.webp "Anti-IKK beta antibody [10AG2] used in IHC (Paraffin sections) (IHC-P). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139](/upload/website/prouct_img/small/GTX12139/GTX12139_62_FACS_w_23060519_715.webp "Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139](/upload/website/prouct_img/small/GTX12139/GTX12139_927_WB_w_23060519_508.webp "Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139](/upload/website/prouct_img/normal/GTX12139/GTX12139_7_FACS_w_23060519_606.webp "Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139](/upload/website/prouct_img/normal/GTX12139/GTX12139_926_WB_w_23060519_956.webp "Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139](/upload/website/prouct_img/normal/GTX12139/GTX12139_63_FACS_w_23060519_346.webp "Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139](/upload/website/prouct_img/normal/GTX12139/GTX12139_64_FACS_w_23060519_856.webp "Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139")
![Anti-IKK beta antibody [10AG2] used in IHC (Paraffin sections) (IHC-P). GTX12139](/upload/website/prouct_img/normal/GTX12139/GTX12139_390_IHC-P_w_23060519_342.webp "Anti-IKK beta antibody [10AG2] used in IHC (Paraffin sections) (IHC-P). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139](/upload/website/prouct_img/normal/GTX12139/GTX12139_62_FACS_w_23060519_715.webp "Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139")
![Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139](/upload/website/prouct_img/normal/GTX12139/GTX12139_927_WB_w_23060519_508.webp "Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139")