GeneTex
  • Country / Location Selection

United States (US)

IKK beta antibody [10AG2]

Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139
Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139
Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139
Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139
Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139
Anti-IKK beta antibody [10AG2] used in IHC (Paraffin sections) (IHC-P). GTX12139
Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139

Cat No. GTX12139

Host

Mouse

Clonality

Monoclonal

Clone Name

10AG2

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, IP, MS

Reactivity

Human, Mouse
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 - 4 μg/ml
ICC/IF Assay dependent
IHC-P 1:10 - 1:500
FACS 0.25 - 0.5 μg / 10⁶ cells
IP 1:10 - 1:500
MS Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase subunit beta , IKK-beta , IKK2 , IKKB , IMD15 , IMD15A , IMD15B , NFKBIKB

Cellular Localization

Cytoplasm,Nucleus

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139

GTX12139 WB Image

WB analysis of (A) Daudi, B) HeLa, and C) NIH3T3 cell lysate using GTX12139 IKK beta antibody [10AG2].
Dilution : 2 μg/ml

Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139

GTX12139 FACS Image

FACS (Intracellular staining) analysis of HEK293 cells using GTX12139 IKK beta antibody [10AG2].
Red : primary antibody
Green : isotype control
Antibody amount : 0.5μg

Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139

GTX12139 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX12139 IKK beta antibody [10AG2].
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA
Permibilization : 0.1% saponin
Antibody amount : 1μg/100 μl buffer

Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139

GTX12139 FACS Image

FACS analysis of HEK293 cells using GTX12139 IKK beta antibody [10AG2].
Red : primary antibody
Green : isotype control
Antibody amount : 0.1μg

Anti-IKK beta antibody [10AG2] used in Flow cytometry (FACS). GTX12139

GTX12139 FACS Image

FACS (Intracellular staining) analysis of Jurkat cells using GTX12139 IKK beta antibody [10AG2].
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA

Anti-IKK beta antibody [10AG2] used in IHC (Paraffin sections) (IHC-P). GTX12139

GTX12139 IHC-P Image

IHC-P analysis of human gallbladder tissue using GTX12139 IKK beta antibody [10AG2].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0

Anti-IKK beta antibody [10AG2] used in Western Blot (WB). GTX12139

GTX12139 WB Image

WB analysis of HeLa cell lysate using GTX12139 IKK beta antibody [10AG2].

REFERENCE

There are currently no references for IKK beta antibody [10AG2] (GTX12139). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IKK beta antibody [10AG2] (GTX12139). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 289