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IKK beta antibody [GT1216]

Recombinant antibody Orthogonal
Anti-IKK beta antibody [GT1216] used in Western Blot (WB). GTX01197
Anti-IKK beta antibody [GT1216] used in Western Blot (WB). GTX01197
Anti-IKK beta antibody [GT1216] used in IHC (Paraffin sections) (IHC-P). GTX01197

Cat. No. GTX01197

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1216

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all IKK beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human IKK beta.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase subunit beta , IKBKB , IKK2 , IKKB , IKKbeta , IMD15 , NFKBIKB , inhibitor of kappa light polypeptide gene enhancer in Bcells, kinase beta , IKK beta

Cellular Localization

Cytoplasm,Nucleus,Membrane raft

Background

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-IKK beta antibody [GT1216] used in Western Blot (WB). GTX01197

GTX01197 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody [GT1216] (GTX01197) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-IKK beta antibody [GT1216] used in Western Blot (WB). GTX01197

GTX01197 WB Image

WB analysis of various samples using GTX01197 IKK beta antibody [GT1216].
Dilution : 1:1000
Loading : 25μg

Anti-IKK beta antibody [GT1216] used in IHC (Paraffin sections) (IHC-P). GTX01197

GTX01197 IHC-P Image

IHC-P analysis of human lung cancer tissue section using GTX01197 IKK beta antibody [GT1216].
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399