GeneTex
United States (US)

IKK beta antibody

Overexpression
GTX105690_39862_IHC_19011506_534.jpg
GTX105690_39862_WB_B_19011506_492.jpg
GTX105690_IFA_19011506_783.jpg
GTX105690_39862_20180817_WB_R_19011506_324.jpg
GTX105690_39862_20180824_WB_19011506_165.jpg

Cat No. GTX105690

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

A431 , PC-12 , DDDDK-human IKBKB-transfected 293T

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human IKK beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ33771 antibody, FLJ36218 antibody, FLJ38368 antibody, FLJ40509 antibody, IKK-beta antibody, IKK2 antibody, IKKB antibody, MGC131801 antibody, NFKBIKB antibody, IKBKB antibody, IKK-B antibody, I-kappa-B kinase 2 antibody, I-kappa-B-kinase beta antibody, nuclear factor NF-kappa-B inhibitor kinase beta antibody, inhibitor of nuclear factor kappa-B kinase subunit beta antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta antibody

Cellular Localization

Cytoplasm

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX105690_39862_IHC_19011506_534.jpg

GTX105690 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IKK beta(GTX105690) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105690_39862_WB_B_19011506_492.jpg

GTX105690 WB Image

Overexpression

IKK beta antibody detects IKK beta protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of DDDDK-human IKBKB-transfected 293T cells
7.5 % SDS-PAGE
IKK beta antibody (GTX105690) dilution: 1:5000

GTX105690_IFA_19011506_783.jpg

GTX105690 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK beta(GTX105690) antibody at 1:200 dilution.

GTX105690_39862_20180817_WB_R_19011506_324.jpg

GTX105690 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody (GTX105690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105690_39862_20180824_WB_19011506_165.jpg

GTX105690 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody (GTX105690) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169