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IKK beta antibody

Overexpression
Anti-IKK beta antibody used in Western Blot (WB). GTX105690
Anti-IKK beta antibody used in Western Blot (WB). GTX105690
Anti-IKK beta antibody used in IHC (Paraffin sections) (IHC-P). GTX105690
Anti-IKK beta antibody used in Western Blot (WB). GTX105690
Anti-IKK beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105690

Cat. No. GTX105690

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IKK beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

DDDDK-human IKBKB-transfected 293T , PC-12 , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human IKK beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950563

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase subunit beta , IKK-beta , IKK2 , IKKB , IMD15 , IMD15A , IMD15B , NFKBIKB

Cellular Localization

Cytoplasm

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IKK beta antibody used in Western Blot (WB). GTX105690

GTX105690 WB Image

Overexpression

IKK beta antibody detects IKK beta protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of DDDDK-human IKBKB-transfected 293T cells
7.5 % SDS-PAGE
IKK beta antibody (GTX105690) dilution: 1:5000

Anti-IKK beta antibody used in Western Blot (WB). GTX105690

GTX105690 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody (GTX105690) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IKK beta antibody used in IHC (Paraffin sections) (IHC-P). GTX105690

GTX105690 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IKK beta(GTX105690) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IKK beta antibody used in Western Blot (WB). GTX105690

GTX105690 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody (GTX105690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKK beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105690

GTX105690 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK beta(GTX105690) antibody at 1:200 dilution.

REFERENCE

Application Reference

Bulati M et al. Front Immunol 2020; 11 : 54 The Immunomodulatory Properties of the Human Amnion-Derived Mesenchymal Stromal/Stem Cells Are Induced by INF-γ Produced by Activated Lymphomonocytes and Are Mediated by Cell-To-Cell Contact and Soluble Factors.
Application : WB
Wu YJ et al. J Immunol 2015; 195 (6) : 2612-23 Cellular FLIP Inhibits Myeloid Cell Activation by Suppressing Selective Innate Signaling.
Application : WB
Reactivity : Mouse
Heng-Hsiung Wu et al. J Exp Med 2015; 212 (3) : 333-49 Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169