Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK beta(GTX105690) antibody at 1:200 dilution.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody (GTX105690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IKK beta(GTX105690) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody (GTX105690) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

IKK beta antibody detects IKK beta protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of DDDDK-human IKBKB-transfected 293T cells
7.5 % SDS-PAGE
IKK beta antibody (GTX105690) dilution: 1:5000