GeneTex
United States (US)

IKK beta antibody

GTX107970_39988_20141128_IHC_19011507_586.jpg
GTX107970_39988_IFA_19011507_584.jpg
GTX107970_39988_20151204_IP_19011507_813.jpg
GTX107970_39988_WB_19011507_780.jpg
GTX107970_39988_20180525_WB_B_19011507_819.jpg

Cat No. GTX107970

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1 , NCI-H929 , DDDDK-tagged IKBKB-transfected 293T

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IKK beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Beta,Ikk-Beta,Ikk2,Ikkb,Imd15,Nfkbikb,Ikbkb

Cellular Localization

Cytoplasm

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX107970_39988_20141128_IHC_19011507_586.jpg

GTX107970 IHC-P Image

IKK beta antibody detects IKK beta protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IKK beta antibody (GTX107970) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107970_39988_IFA_19011507_584.jpg

GTX107970 ICC/IF Image

IKK beta antibody detects IKK beta protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IKK beta protein stained by IKK beta antibody (GTX107970) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX107970_39988_20151204_IP_19011507_813.jpg

GTX107970 IP Image

Immunoprecipitation of IKK beta protein from A431 whole cell extracts using 5 μg of IKK beta antibody (GTX107970).
Western blot analysis was performed using IKK beta antibody (GTX107970).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX107970_39988_WB_19011507_780.jpg

GTX107970 WB Image

IKK beta antibody detects IKBKB protein by Western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg K562 whole cell lysate/extract
D. 30 µg THP-1 whole cell lysate/extract
E. 30 µg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
IKK beta antibody (GTX107970) dilution: 1:1000

GTX107970_39988_20180525_WB_B_19011507_819.jpg

GTX107970 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody (GTX107970) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169