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IKK beta antibody

Overexpression
Anti-IKK beta antibody used in Western Blot (WB). GTX107970
Anti-IKK beta antibody used in Immunoprecipitation (IP). GTX107970
Anti-IKK beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107970
Anti-IKK beta antibody used in Western Blot (WB). GTX107970
Anti-IKK beta antibody used in IHC (Paraffin sections) (IHC-P). GTX107970

Cat. No. GTX107970

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, PLA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IKK beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1 , NCI-H929 , DDDDK-tagged IKBKB-transfected 293T

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IKK beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037238

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase subunit beta , IKK-beta , IKK2 , IKKB , IMD15 , IMD15A , IMD15B , NFKBIKB

Cellular Localization

Cytoplasm

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IKK beta antibody used in Western Blot (WB). GTX107970

GTX107970 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody (GTX107970) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKK beta antibody used in Immunoprecipitation (IP). GTX107970

GTX107970 IP Image

Immunoprecipitation of IKK beta protein from A431 whole cell extracts using 5 μg of IKK beta antibody (GTX107970).
Western blot analysis was performed using IKK beta antibody (GTX107970) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-IKK beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107970

GTX107970 ICC/IF Image

IKK beta antibody detects IKK beta protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IKK beta protein stained by IKK beta antibody (GTX107970) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-IKK beta antibody used in Western Blot (WB). GTX107970

GTX107970 WB Image

IKK beta antibody detects IKBKB protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg THP-1 whole cell lysate/extract
E. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
IKK beta antibody (GTX107970) dilution: 1:1000

Anti-IKK beta antibody used in IHC (Paraffin sections) (IHC-P). GTX107970

GTX107970 IHC-P Image

IKK beta antibody detects IKK beta protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IKK beta antibody (GTX107970) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chou LY et al. Cells 2022; 11 (20) Paracrine Senescence of Mesenchymal Stromal Cells Involves Inflammatory Cytokines and the NF-κB Pathway.
Application : WB
Reactivity : Human
Smaldone G et al. Sci Rep 2021; 11 (1) : 18237 KCTD15 deregulation is associated with alterations of the NF-κB signaling in both pathological and physiological model systems.
Application : IP, PLA
Reactivity : Human
Sharma RK et al. Immunol Invest 2017; 46 (8) : 833-846 Immunomodulatory activities of curcumin-stabilized silver nanoparticles: Efficacy as an antiretroviral therapeutic.
Application : ICC/IF
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169