WB analysis of HeLa whole cell lysate using GTX27611 IKK beta antibody.
Dilution : 1:500

IHC-P analysis of human placenta tissue using GTX27611 IKK beta antibody.
Dilution : 1:500

Western blot of anti-IKKs antibody (GTX27611) reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat anti Rabbit IgG (H&L)) diluted 1:2,000 for 1h. Washes with TBS preceded color development.

GeneTex's Anti-IKKs antibody was diluted 1:500 to detect IKKs in human placenta tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.