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IKK beta antibody

Anti-IKK beta antibody used in IHC (Paraffin sections) (IHC-P). GTX27611
Anti-IKK beta antibody used in Western Blot (WB). GTX27611
Anti-IKK beta antibody used in IHC (Paraffin sections) (IHC-P). GTX27611
Anti-IKK beta antibody used in Western Blot (WB). GTX27611

Cat. No. GTX27611

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($589)
See all IKK beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:1000
IHC-P 1:1:200-1:1000
ELISA 1:1000-1:5000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filtered-steriled serum

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

80 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IKKb peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase subunit beta , IKK-beta , IKK2 , IKKB , IMD15 , IMD15A , IMD15B , NFKBIKB

Cellular Localization

Cytoplasm,Nucleus

Background

NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFk B is negatively modulated by the inhibitory proteins Ik-B. In turn Ik-B is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK-a and IKK-b, which phosphorylate Ik-B leading to its degradation and the resulting translocation of NFk B to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF-a , endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.

Database

Research Area

DATA IMAGES

Anti-IKK beta antibody used in IHC (Paraffin sections) (IHC-P). GTX27611

GTX27611 IHC-P Image

GeneTex's Anti-IKKs antibody was diluted 1:500 to detect IKKs in human placenta tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Anti-IKK beta antibody used in Western Blot (WB). GTX27611

GTX27611 WB Image

Western blot of anti-IKKs antibody (GTX27611) reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat anti Rabbit IgG (H&L)) diluted 1:2,000 for 1h. Washes with TBS preceded color development.

Anti-IKK beta antibody used in IHC (Paraffin sections) (IHC-P). GTX27611

GTX27611 IHC-P Image

IHC-P analysis of human placenta tissue using GTX27611 IKK beta antibody.
Dilution : 1:500

Anti-IKK beta antibody used in Western Blot (WB). GTX27611

GTX27611 WB Image

WB analysis of HeLa whole cell lysate using GTX27611 IKK beta antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589