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IKK beta antibody

Anti-IKK beta antibody used in Western Blot (WB). GTX31645
Anti-IKK beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31645
Anti-IKK beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31645

Cat No. GTX31645

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , Jurkat Cell Lysate(GTX27899) , HeLa Cell Lysate

Specificity/Sensitivity

This polyclonal antibody has no cross response to IKKa or IKKy.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid.The immunogen is located within amino acids 650 - 700 of IKK beta.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase subunit beta , IKK-beta , IKK2 , IKKB , IMD15 , IMD15A , IMD15B , NFKBIKB

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-IKK beta antibody used in Western Blot (WB). GTX31645

GTX31645 WB Image

WB analysis of Jurkat cell lysate using GTX31645 IKK beta antibody.
Working concentration : 1:500

Anti-IKK beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31645

GTX31645 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31645 IKK beta antibody.
Working concentration : 10 μg/ml

Anti-IKK beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31645

GTX31645 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31645 IKK beta antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339