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IKK beta (phospho Tyr199) antibody

Anti-IKK beta (phospho Tyr199) antibody used in Western Blot (WB). GTX55109
Anti-IKK beta (phospho Tyr199) antibody used in IHC (Paraffin sections) (IHC-P). GTX55109

Cat No. GTX55109

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate pH7.3, 0.87% Sodium Chloride, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of IKK beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887915

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase subunit beta , IKK-beta , IKK2 , IKKB , IMD15 , IMD15A , IMD15B , NFKBIKB

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-IKK beta (phospho Tyr199) antibody used in Western Blot (WB). GTX55109

GTX55109 WB Image

WB analysis of LPS-treated HeLa (A), LPS-treated Raw264.7 (B), LPS-treated PC12 (C) whole cell lysates using GTX55109 IKK beta (phospho Tyr199) antibody.

Anti-IKK beta (phospho Tyr199) antibody used in IHC (Paraffin sections) (IHC-P). GTX55109

GTX55109 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55109 IKK beta (phospho Tyr199) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339