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IKK epsilon antibody [C2C3], C-term

Anti-IKK epsilon antibody [C2C3], C-term used in Western Blot (WB). GTX111350
Anti-IKK epsilon antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111350
Anti-IKK epsilon antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111350
Anti-IKK epsilon antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111350

Cat. No. GTX111350

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IKK epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human IKK epsilon. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950564

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase subunit epsilon , IKK-E , IKK-i , IKKE , IKKI

Cellular Localization

Cytoplasm

Background

IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IKK epsilon antibody [C2C3], C-term used in Western Blot (WB). GTX111350

GTX111350 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX111350 diluted at 1:1000

Anti-IKK epsilon antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111350

GTX111350 IHC-P Image

IKBKE antibody [C2C3], C-term detects IKBKE protein at cytoplasm in human endometrium by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrium.
IKBKE antibody [C2C3], C-term (GTX111350) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IKK epsilon antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111350

GTX111350 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using IKBKE(GTX111350) antibody at 1:200 dilution.

Anti-IKK epsilon antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111350

GTX111350 ICC/IF Image

IKBKE antibody [C2C3], C-term detects IKBKE protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: IKBKE protein stained by IKBKE antibody [C2C3], C-term (GTX111350) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169