GeneTex
United States (US)

IKK epsilon antibody

Cat No. GTX27891

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

80 kDa. ( Note )

Positive Control

Jurkat whole cell lysate

Specificity/Sensitivity

IKK epsilon - Does not cross react with IKK alpha, beta or gamma
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide: amino acids 705-716 of human IKK epsilon

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Epsilon,Ikk-E,Ikk-I,Ikke,Ikki,Ikbke

Background

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase(IKK) complex that contains IKKa, IKKb, and IKKg. A novel molecule in the IKK complex was recently identified and designated IKKe/IKK-i (1,2). IKKe is required for the activation of NF-kB by PMA and T cell receptors but not by TNFa and IL-1 (1). IKKe/IKK-i message is expressed in a variety of tissues and is inducible by TNFa, IL-1, and LPS (2).

Database

Research Area

Package List Price ($)
$ 329