APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human IKK gamma. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
inhibitor of nuclear factor kappa B kinase regulatory subunit gamma , AMCBX1 , EDAID1 , FIP-3 , FIP3 , Fip3p , IKK-gamma , IKKAP1 , IKKG , IMD33 , IP , IP1 , IP2 , IPD2 , NEMO , ZC2HC9
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]
Database
Research Area
DATA IMAGES
![Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052](/templates/default/js/lightbox/images/loading.gif "Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052")
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GTX637052 WB Image
Whole cell extract (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (GTX637052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637052 IHC-P Image
IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.IKK gamma stained by IKK gamma antibody [HL1577] (GTX637052) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637052 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (GTX637052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637052 ICC/IF Image
IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IKK gamma stained by IKK gamma antibody [HL1577] (GTX637052) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX637052 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (GTX637052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for IKK gamma antibody [HL1577] (GTX637052). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IKK gamma antibody [HL1577] (GTX637052). Be the first to share your experience with this product.
![Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052](/upload/website/prouct_img/small/GTX637052/GTX637052_T-44711_20220708_WB_22071401_758.webp "Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052")
![Anti-IKK gamma antibody [HL1577] used in IHC (Paraffin sections) (IHC-P). GTX637052](/upload/website/prouct_img/small/GTX637052/GTX637052_T-44711_20220708_IHC-P_M_22071823_745.webp "Anti-IKK gamma antibody [HL1577] used in IHC (Paraffin sections) (IHC-P). GTX637052")
![Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052](/upload/website/prouct_img/small/GTX637052/GTX637052_T-44711_20220715_WB_22071823_101.webp "Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052")
![Anti-IKK gamma antibody [HL1577] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637052](/upload/website/prouct_img/small/GTX637052/GTX637052_T-44711_20220916_ICC_IF_22102723_624.webp "Anti-IKK gamma antibody [HL1577] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637052")
![Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052](/upload/website/prouct_img/small/GTX637052/GTX637052_T-44711_20220819_WB_M_22082402_411.webp "Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052")
![Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052](/upload/website/prouct_img/normal/GTX637052/GTX637052_T-44711_20220708_WB_22071401_758.webp "Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052")
![Anti-IKK gamma antibody [HL1577] used in IHC (Paraffin sections) (IHC-P). GTX637052](/upload/website/prouct_img/normal/GTX637052/GTX637052_T-44711_20220708_IHC-P_M_22071823_745.webp "Anti-IKK gamma antibody [HL1577] used in IHC (Paraffin sections) (IHC-P). GTX637052")
![Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052](/upload/website/prouct_img/normal/GTX637052/GTX637052_T-44711_20220715_WB_22071823_101.webp "Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052")
![Anti-IKK gamma antibody [HL1577] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637052](/upload/website/prouct_img/normal/GTX637052/GTX637052_T-44711_20220916_ICC_IF_22102723_624.webp "Anti-IKK gamma antibody [HL1577] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637052")
![Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052](/upload/website/prouct_img/normal/GTX637052/GTX637052_T-44711_20220819_WB_M_22082402_411.webp "Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052")