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IKK gamma antibody [HL1577]

Recombinant antibody
Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052
Anti-IKK gamma antibody [HL1577] used in IHC (Paraffin sections) (IHC-P). GTX637052
Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052
Anti-IKK gamma antibody [HL1577] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637052
Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052

Cat No. GTX637052

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1577

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IKK gamma. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase regulatory subunit gamma , AMCBX1 , EDAID1 , FIP-3 , FIP3 , Fip3p , IKK-gamma , IKKAP1 , IKKG , IMD33 , IP , IP1 , IP2 , IPD2 , NEMO , ZC2HC9

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052

GTX637052 WB Image

Whole cell extract (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (GTX637052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKK gamma antibody [HL1577] used in IHC (Paraffin sections) (IHC-P). GTX637052

GTX637052 IHC-P Image

IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
IKK gamma stained by IKK gamma antibody [HL1577] (GTX637052) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052

GTX637052 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (GTX637052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKK gamma antibody [HL1577] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637052

GTX637052 ICC/IF Image

IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IKK gamma stained by IKK gamma antibody [HL1577] (GTX637052) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-IKK gamma antibody [HL1577] used in Western Blot (WB). GTX637052

GTX637052 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (GTX637052) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations