GeneTex
United States (US)

IKK gamma antibody

IKK-gamma-antibody_WB_GTX27890-1_18121411_857.jpg

Cat No. GTX27890

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

48 kDa. ( Note )

Positive Control

Hela whole cell lysate

Specificity/Sensitivity

IKK gamma - Does not cross react with IKK alpha, beta or epsilon
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide: amino acids 400-416 of human IKK gamma. This sequence is identical to that of mouse.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Gamma,Amcbx1,Fip-3,Fip3,Fip3P,Ikk-Gamma,Ikkap1,Ikkg,Imd33,Ip,Ip1,Ip2,Ipd2,Nemo,Zc2Hc9,Ikbkg

Background

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The IkB kinase (IKKa and IKKb)phosphorylates IkB and mediates NF-kB activation. A novel molecule in the IKK complex was recently identified and termed IKKg/NEMO/FIP3/IKKAP1 (1-5). IKKg interacts with IKKb and is required for the activation of IKK complex and NF-kB by LPS, PMA, TNF, and IL-1 stimulation (1-4). FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kB activation (3).

Database

Research Area

DATA IMAGES
IKK-gamma-antibody_WB_GTX27890-1_18121411_857.jpg

GTX27890 WB Image

Western blot analysis of IKKg in HeLa whole cell lysate with Anti-IKKg (Product No. GTX27890) at 1 μg/ml.

Package List Price ($)
$ 389