For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.
Hela whole cell lysate
IKK gamma - Does not cross react with IKK alpha, beta or epsilon
Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide: amino acids 400-416 of human IKK gamma. This sequence is identical to that of mouse.
Affinity purified with antigen
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Gamma,Amcbx1,Fip-3,Fip3,Fip3P,Ikk-Gamma,Ikkap1,Ikkg,Imd33,Ip,Ip1,Ip2,Ipd2,Nemo,Zc2Hc9,Ikbkg
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The IkB kinase (IKKa and IKKb)phosphorylates IkB and mediates NF-kB activation. A novel molecule in the IKK complex was recently identified and termed IKKg/NEMO/FIP3/IKKAP1 (1-5). IKKg interacts with IKKb and is required for the activation of IKK complex and NF-kB by LPS, PMA, TNF, and IL-1 stimulation (1-4). FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kB activation (3).
GTX27890 WB Image
Western blot analysis of IKKg in HeLa whole cell lysate with Anti-IKKg (Product No. GTX27890) at 1 μg/ml.