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IKK gamma antibody

KO/KD
Anti-IKK gamma antibody used in Western Blot (WB). GTX35211
Anti-IKK gamma antibody used in Western Blot (WB). GTX35211
Anti-IKK gamma antibody used in Immunoprecipitation (IP). GTX35211

Cat. No. GTX35211

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all IKK gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human IKBKG (NP_003630.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of nuclear factor kappa B kinase regulatory subunit gamma , AMCBX1 , EDAID1 , FIP-3 , FIP3 , Fip3p , IKK-gamma , IKKAP1 , IKKG , IMD33 , IP , IP1 , IP2 , IPD2 , NEMO , ZC2HC9

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-IKK gamma antibody used in Western Blot (WB). GTX35211

GTX35211 WB Image

WB analysis of various sample lysates using GTX35211 IKK gamma antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IKK gamma antibody used in Western Blot (WB). GTX35211

GTX35211 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35211 IKK gamma antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IKK gamma antibody used in Immunoprecipitation (IP). GTX35211

GTX35211 IP Image

IP analysis of A549 cell lysate using GTX35211 IKK gamma antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399