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IKK gamma antibody

Anti-IKK gamma antibody used in Western Blot (WB). GTX70362

Cat No. GTX70362

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
100 μg ($289)

APPLICATION

Application Note

Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Specificity/Sensitivity

Reacts with the residues 2-13 [NRHLWKSQLCEM] of the IKK-g protein. Recognizes both human isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS containing 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Affinity purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Pro-inflammatory cytokines activate the transcription factor NF-kappaB by stimulating the activity of a protein kinase that phosphorylates IkappaB, an inhibitor of NF-kappaB, at sites that trigger its ubiquitination and degradation. A large, cytokine-responsive IkappaB kinase (IKK) complex contains 2 subunits, IKK-alpha and IKK-beta, which are protein kinases whose function is needed for NF-kappaB activation by pro-inflammatory stimuli. IKK is composed of similar amounts of IKK-alpha, IKK-beta, which are differentially processed forms of a third subunit, IKK-gamma. IKK-gamma interacts preferentially with IKK-beta and is required for the activation of the IKK complex.

Research Area

DATA IMAGES

Anti-IKK gamma antibody used in Western Blot (WB). GTX70362

GTX70362 WB Image

Western blot analysis of IKK-g in Interlukin-1-stimulated 293 cells.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 289