APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa (starvation 16 hr hTNF-alpha 20 ng/ml and 100 nM Calyculin A treatment for 15 min)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.68 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser376 of human IKK gamma. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
BSA|Glycerol|PBS|ProClin
Conjugation
Unconjugated
RRID
AB_2887305
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
inhibitor of nuclear factor kappa B kinase regulatory subunit gamma , AMCBX1 , EDAID1 , FIP-3 , FIP3 , Fip3p , IKK-gamma , IKKAP1 , IKKG , IMD33 , IP , IP1 , IP2 , IPD2 , NEMO , ZC2HC9
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]
Database
Research Area
DATA IMAGES
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GTX134550 IHC-P Image
IKK gamma (phospho Ser376) antibody detects IKK gamma (phospho Ser376) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.IKK gamma (phospho Ser376) stained by IKK gamma (phospho Ser376) antibody (GTX134550) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134550 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma (phospho Ser376) antibody (GTX134550) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for IKK gamma (phospho Ser376) antibody (GTX134550). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IKK gamma (phospho Ser376) antibody (GTX134550). Be the first to share your experience with this product.
