GeneTex

  • Country / Location Selection

United States (US)

IKZF4 antibody

Anti-IKZF4 antibody used in Western Blot (WB). GTX128043
Anti-IKZF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX128043

Cat. No. GTX128043

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IKZF4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

Raji , K562

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IKZF4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885689

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IKAROS family zinc finger 4 , EOS , ZNFN1A4

Cellular Localization

Nucleus

Background

Members of the Ikaros (ZNFN1A1; MIM 603023) family of transcription factors, which includes Eos, are expressed in lymphocytes and are implicated in the control of lymphoid development.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IKZF4 antibody used in Western Blot (WB). GTX128043

GTX128043 WB Image

IKZF4 antibody detects IKZF4 protein by western blot analysis.
A. 30 μg Raji whole cell lysate/extract
B. 30 μg K562 whole cell lysate/extract
7.5% SDS-PAGE
IKZF4 antibody (GTX128043) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IKZF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX128043

GTX128043 IHC-P Image

IKZF4 antibody detects IKZF4 protein at cytosol and nucleus on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
IKZF4 antibody (GTX128043) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Yamanaka S et al. Nat Commun 2023; 14 (1) : 4683 Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation.
Submit a Reference

REVIEW

There are currently no reviews for IKZF4 antibody (GTX128043). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169