GeneTex
United States (US)

IL1 Receptor 2 antibody [N3C3]

GTX108702_40016_20150420_IHC_2_19011507_332.jpg
GTX108702_40016_IFA_19011507_255.jpg
GTX108702_WB_19011507_111.jpg
GTX108702_40016_20150420_IHC_19011507_424.jpg
GTX108702_40016_20170929_WB_M_R_19011507_847.jpg

Cat No. GTX108702

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , H1299 , IL1R2 transfected Ad293 cell , soluble IL1R2 transfected Ad293 cell , mouse brain , rat brain

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 Receptor 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 1 Receptor Type 2,Cd121B,Cdw121B,Il-1R-2,Il-1Rt-2,Il-1Rt2,Il1R2C,Il1Rb,Il1R2

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108702_40016_20150420_IHC_2_19011507_332.jpg

GTX108702 IHC-P Image

IL1 Receptor 2 antibody [N3C3] detects IL1 Receptor 2 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108702_40016_IFA_19011507_255.jpg

GTX108702 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using IL1 Receptor 2(GTX108702) antibody at 1:500 dilution.

GTX108702_WB_19011507_111.jpg

GTX108702 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX108702 diluted at 1:1000

GTX108702_40016_20150420_IHC_19011507_424.jpg

GTX108702 IHC-P Image

IL1 Receptor 2 antibody [N3C3] detects IL1 Receptor 2 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108702_40016_20170929_WB_M_R_19011507_847.jpg

GTX108702 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169