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IL1 Receptor 2 antibody [N3C3]

Orthogonal
Anti-IL1 Receptor 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108702
Anti-IL1 Receptor 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108702
Anti-IL1 Receptor 2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108702
Anti-IL1 Receptor 2 antibody [N3C3] used in Western Blot (WB). GTX108702
Anti-IL1 Receptor 2 antibody [N3C3] used in Western Blot (WB). GTX108702

Cat. No. GTX108702

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IL1 Receptor 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , mouse brain , rat brain

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 Receptor 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950579

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 receptor type 2 , CD121b , CDw121b , IL-1R-2 , IL-1RT-2 , IL-1RT2 , IL1R2c , IL1RB

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL1 Receptor 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108702

GTX108702 IHC-P Image

IL1 Receptor 2 antibody [N3C3] detects IL1 Receptor 2 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 Receptor 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108702

GTX108702 IHC-P Image

IL1 Receptor 2 antibody [N3C3] detects IL1 Receptor 2 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 Receptor 2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108702

GTX108702 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using IL1 Receptor 2(GTX108702) antibody at 1:500 dilution.

Anti-IL1 Receptor 2 antibody [N3C3] used in Western Blot (WB). GTX108702

GTX108702 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 Receptor 2 antibody [N3C3] used in Western Blot (WB). GTX108702

GTX108702 WB Image

Orthogonal

LoVo whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Mar AC et al. J Biol Chem 2015; 290 (36) : 22212-24 Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169