LoVo whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A549, using IL1 Receptor 2(GTX108702) antibody at 1:500 dilution.

IL1 Receptor 2 antibody [N3C3] detects IL1 Receptor 2 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IL1 Receptor 2 antibody [N3C3] detects IL1 Receptor 2 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
IL1 Receptor 2 antibody [N3C3] (GTX108702) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min