APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Neuro2A whole , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12
Predict Reactivity
Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.86 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human IL1 Receptor antagonist. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950581
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 1 receptor antagonist , DIRA , ICIL-1RA , IL-1RN , IL-1ra , IL-1ra3 , IL1F3 , IL1RA , IRAP , MVCD4
Cellular Localization
Isoform 1: Secreted , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 4: Cytoplasm
Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX106490 IHC-P Image
Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist(GTX106490) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX106490 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL1 Receptor antagonist antibody (GTX106490) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX106490 WB Image
IL1 Receptor antagonist antibody detects IL1 Receptor antagonist protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with IL1 Receptor antagonist antibody (GTX106490) diluted by 1:1000
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GTX106490 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist (GTX106490) antibody at 1:200 dilution.
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GTX106490 WB Image
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis.
A. 30 μg Neuro2A whole lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
15% SDS-PAGE
IL1 Receptor antagonist antibody (GTX106490) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106490 WB Image
IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
15% SDS-PAGE
IL1 Receptor antagonist antibody (GTX106490) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
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