GeneTex
United States (US)

IL1 Receptor antagonist antibody

GTX106490_39995_WB_19011506_344.jpg
GTX106490_39995_WB_M_19011506_724.jpg
GTX106490_IFA_19011506_818.jpg
GTX106490_39995_IHC_19011506_813.jpg
GTX106490_39995_WB_R_19011506_908.jpg

Cat No. GTX106490

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Raji , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 Receptor antagonist. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DIRA antibody, ICIL-1RA antibody, IL-1RN antibody, IL-1ra antibody, IL-1ra3 antibody, IL1F3 antibody, IL1RA antibody, IRAP antibody, MGC10430 antibody, MVCD4 antibody, IL1RN antibody, IL1 inhibitor antibody, IL1RN (IL1F3) antibody, interleukin-1 receptor antagonist protein antibody, intracellular IL-1 receptor antagonist type II antibody, type II interleukin-1 receptor antagonist antibody, intracellular interleukin-1 receptor antagonist (icIL-1ra) antibody, interleukin 1 receptor antagonist antibody

Cellular Localization

Isoform 1: Secreted , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 4: Cytoplasm

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106490_39995_WB_19011506_344.jpg

GTX106490 WB Image

IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis.
A. 30 µg Raji whole cell lysate/extract
15% SDS-PAGE
IL1 Receptor antagonist antibody (GTX106490) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106490_39995_WB_M_19011506_724.jpg

GTX106490 WB Image

IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis.
A. 30 µg Neuro2A whole lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg Raw264.7 whole cell lysate/extract
E. 30 µg C2C12 whole cell lysate/extract
15% SDS-PAGE
IL1 Receptor antagonist antibody (GTX106490) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106490_IFA_19011506_818.jpg

GTX106490 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist (GTX106490) antibody at 1:200 dilution.

GTX106490_39995_IHC_19011506_813.jpg

GTX106490 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist(GTX106490) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106490_39995_WB_R_19011506_908.jpg

GTX106490 WB Image

IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
15% SDS-PAGE
IL1 Receptor antagonist antibody (GTX106490) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf