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IL1 Receptor antagonist antibody

Anti-IL1 Receptor antagonist antibody used in Western Blot (WB). GTX106490
Anti-IL1 Receptor antagonist antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106490
Anti-IL1 Receptor antagonist antibody used in IHC (Paraffin sections) (IHC-P). GTX106490
Anti-IL1 Receptor antagonist antibody used in Western Blot (WB). GTX106490
Anti-IL1 Receptor antagonist antibody used in Western Blot (WB). GTX106490
Anti-IL1 Receptor antagonist antibody used in Western Blot (WB). GTX106490

Cat No. GTX106490

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Neuro2A whole , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 Receptor antagonist. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950581

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 receptor antagonist , DIRA , ICIL-1RA , IL-1RN , IL-1ra , IL-1ra3 , IL1F3 , IL1RA , IRAP , MVCD4

Cellular Localization

Isoform 1: Secreted , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 4: Cytoplasm

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL1 Receptor antagonist antibody used in Western Blot (WB). GTX106490

GTX106490 WB Image

IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis.
A. 30 μg Neuro2A whole lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
15% SDS-PAGE
IL1 Receptor antagonist antibody (GTX106490) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 Receptor antagonist antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106490

GTX106490 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist (GTX106490) antibody at 1:200 dilution.

Anti-IL1 Receptor antagonist antibody used in IHC (Paraffin sections) (IHC-P). GTX106490

GTX106490 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist(GTX106490) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 Receptor antagonist antibody used in Western Blot (WB). GTX106490

GTX106490 WB Image

IL1 Receptor antagonist antibody detects IL1RN protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
15% SDS-PAGE
IL1 Receptor antagonist antibody (GTX106490) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 Receptor antagonist antibody used in Western Blot (WB). GTX106490

GTX106490 WB Image

IL1 Receptor antagonist antibody detects IL1 Receptor antagonist protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with IL1 Receptor antagonist antibody (GTX106490) diluted by 1:1000

Anti-IL1 Receptor antagonist antibody used in Western Blot (WB). GTX106490

GTX106490 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL1 Receptor antagonist antibody (GTX106490) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169